Isotype Control (IsoC), Western Blotting (WB), Immunoprecipitation (IP)
Aufreinigung
The antibodies are purified by affinity chromatography, and conjugated with Alexa Fluor® 700 under optimal conditions. The solutions are free of unconjugated Alexa Fluor® 700.
Optimal working dilution should be determined by the investigator.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Buffer
This two-reagent set contains Alexa Fluor® 700 conjugated antibodies and isotype controls in optimal concentration for flow cytometric analysis.
Handhabung
Protect from prolonged exposure to light. Do not freeze.
Lagerung
4 °C
Informationen zur Lagerung
The antibody solution should be stored undiluted between 2°C and 8°C.
Target
IgG2a,IgG2b
Andere Bezeichnung
IgG2b,IgG2a
Hintergrund
The mouse lineage panel has been designed to react with cells from the major hematopoietic cell lineages, such as T lymphocytes, B lymphocytes, monocytes/macrophages, granulocytes, NK cells, and erythrocytes. The Alexa Fluor® 700 Mouse Lineage Antibody Cocktail is designed for the flow cytometric identification of hematopoietic progenitors in mouse bone marrow. Components include anti-mouse CD3, clone 17A2, anti-mouse Ly-6G/Ly-6C, clone RB6-8C5, anti-mouse CD11b, clone M1/70, anti-mouse CD45R/B220, clone RA3-6B2, anti-mouse TER-119/Erythroid cells, clone Ter-119. Alexa Fluor® 700 Mouse Lineage Isotype Control Cocktail contains equivalent concentrations of isotype-matched negative control immunoglobulin. Materials provided: 1. Alexa Fluor® 700 anti-mCD3/ Alexa Fluor® 700 anti-mLy-6G(Ly-6C)/ Alexa Fluor® 700 anti-mCD11b/ Alexa Fluor® 700 anti-mCD45R(B220)/ Alexa Fluor® 700 anti-mTer-119 2. Alexa Fluor® 700 Rat IgG2b/Rat IgG2a isotype controls Materials not provided: Cell Staining Buffer (Cat. No. 420201)