CDC42 Antikörper (AA 1-191)

Produktnummer ABIN968255

Produktdetails anti-CDC42 Antikörper

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Bindungsspezifität: AA 1-191

Reaktivität: Human, Maus, Ratte, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser CDC42 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF)

Kreuzreaktivität: Ratte (Rattus), Hund, Maus

Produktmerkmale: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CDC42 aa. 1-191

Klon: 44-CDC42

Isotyp: IgG1

Anwendungsinformationen

Kommentare:

Related Products: ABIN967389, ABIN968545

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: Store undiluted at -20°C.

Referenzen für anti-CDC42 Antikörper (ABIN968255)

Habas, Dawid, He: "Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation." in: Genes & development, Vol. 17, Issue 2, pp. 295-309, (2003) (PubMed).

Brandt, Gimona, Hillmann, Haller, Mischak: "Protein kinase C induces actin reorganization via a Src- and Rho-dependent pathway." in: The Journal of biological chemistry, Vol. 277, Issue 23, pp. 20903-10, (2002) (PubMed).

Zugasti, Rul, Roux, Peyssonnaux, Eychene, Franke, Fort, Hibner: "Raf-MEK-Erk cascade in anoikis is controlled by Rac1 and Cdc42 via Akt." in: Molecular and cellular biology, Vol. 21, Issue 19, pp. 6706-17, (2001) (PubMed).

Details zu CDC42

Target: CDC42 (Cell Division Cycle 42 (GTP Binding Protein, 25kDa) (CDC42))

Andere Bezeichnung: Cdc42 (CDC42 Produkte)

Synonyme: CDC42Hs antikoerper, G25K antikoerper, AI747189 antikoerper, AU018915 antikoerper, cdc42a antikoerper, wu:fb07d08 antikoerper, wu:fj62g06 antikoerper, zgc:63918 antikoerper, Xcdc42 antikoerper, cdc42hs antikoerper, g25k antikoerper, CDC42 antikoerper, CG12530 antikoerper, Cdc42Dm antikoerper, D-CDC42 antikoerper, D-Cdc42 antikoerper, D-cdc42 antikoerper, DCDC42 antikoerper, DCdc42 antikoerper, Dcdc42 antikoerper, Dm Cdc42 antikoerper, DmCDC42 antikoerper, Dmcdc42 antikoerper, Dmel\\CG12530 antikoerper, cdc42 antikoerper, cell division cycle 42 antikoerper, Cell division control protein 42 homolog antikoerper, cell division control protein 42 homolog antikoerper, cell division cycle 42 L homeolog antikoerper, CG12530 gene product from transcript CG12530-RC antikoerper, Rho family GTPase antikoerper, CDC42 antikoerper, Cdc42 antikoerper, cdc42 antikoerper, LOC100636735 antikoerper, LOC100637699 antikoerper, LOC100638834 antikoerper, cdc42.L antikoerper

Hintergrund: Rho family members are small GTP binding proteins that serve as molecular switches for a number of biological processes. They cycle between active GTP-bound and inactive GDP-bound states. CDC42 is a Rho family protein that was identified in membranes of human platelets and placenta. It is the homologue of CDC42Sc, which regulates initiation of bud-site assembly in Saccharomyces cerevisiae. CDC42 regulates the function of the mammalian actin cytoskeleton, allowing for efficient cytokinesis and cell morphogenesis. CDC42 and Rac1, a Ras-related GTPase, activate MEKK1, a JNK kinase kinase, which leads to the activation of several downstream components of the MAP kinase cascade leading to activation of PAK65, a CDC42- and Rac-binding protein. PAK65 interacts with CDC42/Rac1, mediates their interaction with MEKK1, and enhances MEKK1 catalytic activity. Chronic activation of CDC42 has been shown to induce malignant cellular transformation. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody.

Molekulargewicht: 22 kDa

Pathways: MAPK Signalweg, Microtubule Dynamics, RTK Signalweg, WNT Signalweg, T-Zell Rezeptor Signalweg, EGFR Signaling Pathway, Regulation of Actin Filament Polymerization, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Maintenance of Protein Location, Skeletal Muscle Fiber Development, Signaling Events mediated by VEGFR1 and VEGFR2, EGFR Downregulation, VEGF Signaling