P21/WAF1 Antikörper

Antigen: P21/WAF1

Klonalität: Monoklonal (WA-1)

Wirt: Maus

Reaktivität: Human, Primaten

Applikation: Western Blot (WB), Immunhistochemie (Paraffinschnitte) (IHC (p)), ELISA, Immunzytochemie (ICC)

Produktnummer: ABIN94444

Produktbeschreibung

Weitere Bezeichnung: p21Waf1

Immunogen: Human p21 protein

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: WA-1

Beschreibung: The tumour suppressor p21Waf1 (Wild-type p53-Activated Fragment 1, also known as Cip1,Cdk interacting protein, or SDI 1) is a cyclin-dependent kinase (Cdk) inhibitor, which isexpressed by involvement of p53, Egr-1, AP2, STATs or other transcription factors uponvarious stimuli resulting in cell cycle arrest. Through its N-terminal domain p21Waf1 inhibitsCdk activity, whereas through the C-terminal domain it inhibits the activity of PCNA(proliferating cell nuclear antigen) to activate DNA replication. Cytosolic location of p21counteracts its inhibitory activities.

Spezifität: The antibody WA-1 reacts with p21 protein (Waf1, Cip1, SDI 1), a 21 kDa tumoursuppressor, which inhibits the activity of cyclin-dependent family kinases and of proliferatingcell nuclear antigen.

Anwendungen

Applikationshinweise: Immunohistochemistry (paraffin sections) - Positive tissue: colon carcinomaImmunocytochemistryELISA

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by precipitation methods.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Immunohistochemistry staining of colon carcinoma (paraffin-embedded sections) with anti-p21 (WA-1).