Maus anti-Human IgM (Fc Region) Antikörper

Details zu Produkt Nr. ABIN94402, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Fc Region
561
369
288
63
56
48
45
27
6
6
2
1
1
1
1
Reaktivität
Human
1208
568
236
92
80
67
67
58
56
56
56
40
35
28
19
15
15
7
5
5
2
1
1
1
Wirt
Maus
1266
678
525
144
38
11
10
9
4
3
3
1
Klonalität (Klon)
Monoklonal ()
Applikation
Immunocytochemistry (ICC), Flow Cytometry (FACS), ELISA, Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified human IgM.
Klon CH2
Isotyp IgG1
Spezifität The antibody CH2 reacts with Fc fragment of human IgM.
Reinigung Purified from ascites by precipitation methods and ion exchange chromatography.
Reinheit > 95 % (by SDS-PAGE)
Hintergrund Immunoglobulin M (IgM) is produced as a 900 kDa pentamer, which is an efficient complement binder. This antibody type is produced initially in the immune response and it is the first immunoglobulin class to be synthesized by a fetus or newborn. IgM antibodies do not cross the placenta. IgM concentration in blood is 0.12 g/l and its biological survival (plasma T1/2) is 5 days.
Molekulargewicht 900 kDa
Forschungsgebiet Secondary Antibodies
Applikationshinweise Flow Cytometry: Recommended dilution: 1 µg/mL

Working concentrations should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Flow Cytometry (FACS) image for Mouse anti-Human IgM (Fc Region) antibody (ABIN94402) Surface staining of human peripheral blood cells with anti-human IgM (CH2) APC. Cells...
Allgemeine Veröffentlichungen Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).