Maus anti-Rind (Kuh) IgG (Light Chain) Antikörper

Details zu Produkt Nr. ABIN94401, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Light Chain
4681
2537
1879
1066
1006
859
298
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Rind (Kuh)
2850
2735
2296
1675
1433
788
613
441
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Wirt
Maus
6705
4831
1795
890
609
388
91
55
52
21
8
5
5
5
2
1
1
Klonalität (Klon)
Monoklonal ()
Applikation
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Fraction of Bovine immunoglobulin.
Klon IVA285-1
Isotyp IgG1
Spezifität The antibody IVA285-1 reacts with bovine IgG (light chains). IgG light chains (kappa nad lambda types) consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region.
Reinigung Purified from ascites by protein-A affinity chromatography.
Reinheit > 95 % (by SDS-PAGE)
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise Western Blotting: Recommended dilution: 0,5-1 µg/mL, overnight in 4 °C
Positive control and sample preparation: BS (bovine serum), dilution 1: 50 in Laemmli reducing buffer, boiled in water bath for 3 min
Application notes: Strongly reacts with bovine IgG light chains, weakly reacts with IgM.Reducing conditions. SDS-PAGE (12 % separating gel).
Flow Cytometry:

Working concentrations should be determined by the investigator.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Do not freeze.
Lagerung 4 °C
Informationen zur Lagerung Store at 2-8 °C. Do not use after expiration date stamped on vial label.
Bilder des Herstellers
Western Blotting (WB) image for Mouse anti-Cow (Bovine) IgG (Light Chain) antibody (ABIN94401) Detection of IgG light chain in reduced samples of Fetal Calf Serum (left lane) and B...
Produkt verwendet in: Antalíková, Simon, Jankovicová, Horovská, Dusinský, Hluchý: "Monoclonal antibody to the light chain of bovine immunoglobulin." in: Hybridoma (2005), Vol. 25, Issue 5, pp. 309-12, 2006 (PubMed).