CD14 Molecule (CD14) Antikörper

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (MEM-15)

Wirt: Maus

Reaktivität: Human, Primaten

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP)

Produktnummer: ABIN93971

Produktbeschreibung

Weitere Bezeichnung: CD14

Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from theurine of a patient suffering from proteinuria.

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: MEM-15

Beschreibung: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface ofmature monocytes, macrophages, and neutrophils, where serves as a multifunctionallipopolysaccharide receptor, it is also released to the serum both as a secreted andenzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in areaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serumprotein. The soluble sCD14 is able to discriminate slight structural differences betweenlipopolysaccharides and is important for neutralization of serum allochthonouslipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic,inflammatory and infectious processes.

Spezifität: The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI(glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes,macrophages and weakly on granulocytes, also expressed by most tissue macrophages.The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in theurine of some nephrotic patients.HLDA III, WS Code M 252HLDA IV, WS Code M 113HLDA IV, WS Code NL 90HLDA IV, WS Code T 53HLDA V, WS Code M MA086HLDA VI, WS Code M MA94

Anwendungen

Applikationshinweise: Immunoprecipitation: excellent for immunoprecipitation of CD14

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from ascites by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Bazil, Horejsí, Baudys et al.: "Biochemical characterization of a soluble form of the 53-kDa monocyte surface antigen." in: European journal of immunology, Vol. 16, Issue 12, pp. 1583-9, 1987 (PubMed).

Sundan, Gullstein-Jahr, Otterlei et al.: "Soluble CD14 from urine copurifies with a potent inducer of cytokines." in: European journal of immunology, Vol. 24, Issue 8, pp. 1779-84, 1994 (PubMed).