CD14 Molecule (CD14) Antikörper (FITC)

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (MEM-18)

Wirt: Maus

Reaktivität: Human, Primaten

Konjugat: FITC

Applikation: Durchflusszytometrie (FACS)

Produktnummer: ABIN93966

Produktbeschreibung

Weitere Bezeichnung: CD14

Immunogen: A crude mixture of human urinary proteins precipitated by ammonium sulphate from theurine of a patient suffering from proteinuria.

Isotyp: IgG1

Klon: MEM-18

Beschreibung: CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface ofmature monocytes, macrophages, and neutrophils, where serves as a multifunctionallipopolysaccharide receptor, it is also released to the serum both as a secreted andenzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in areaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serumprotein. The soluble sCD14 is able to discriminate slight structural differences betweenlipopolysaccharides and is important for neutralization of serum allochthonouslipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic,inflammatory and infectious processes.

Spezifität: The antibody MEM-18 reacts with CD14, a 53-55 kDa GPI(glycosylphosphatidylinositol)-linked membrane glycoprotein expressed on monocytes,macrophages and weakly on granulocytes, also expressed by most tissue macrophages. Inhuman, the epitope recognized by MEM-18 is located between amino acids 57-64.HLDA III, WS Code M 253HLDA IV, WS Code M 314HLDA V, WS Code M MA087HLDA VI, WS Code M MA95

Anwendungen

Applikationshinweise: The reagent is designed for Flow Cytometry analysis of human blood cells using 20 µl reagent / 100 µl of whole blood or 10 6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

Buffer: The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azideand 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.

Lagerung: Store in the dark at 2-8 o C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.Short-term exposure to room temperature should not affect the quality of the reagent.However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Western Blotting analysis (non-reducing conditions) of over-expressed human CD14 using anti-CD14 (MEM-18). Lane 1: whole cell lysate HEK 293 transfected with empty vector. Lane 2: tissue culture supernatant collected after cultivation of HEK 293 transfected with human CD14 cDNA. Lane 3: whole cell lysate of HEK 293 transfected with human CD14 cDNA

Publikationen

Publikationen: Stöckl, Majdic, Fischer et al.: "Monomorphic molecules function as additional recognition structures on haptenated target cells for HLA-A1-restricted, hapten-specific CTL." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 5, pp. 2724-33, 2001 (PubMed).

Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).

Angel, Lala, Chen et al.: "CD14+ antigen-presenting cells in human dermis are less mature than their CD1a+ counterparts." in: International immunology, Vol. 19, Issue 11, pp. 1271-9, 2007 (PubMed).

Weiss, Lichtenauer, Kirchner et al.: "Hepatic progenitor cells from adult human livers for cell transplantation." in: Gut, Vol. 57, Issue 8, pp. 1129-38, 2008 (PubMed).

von Schlieffen, Oskolkova, Schabbauer et al.: "Multi-hit inhibition of circulating and cell-associated components of the toll-like receptor 4 pathway by oxidized phospholipids." in: Arteriosclerosis, thrombosis, and vascular biology, Vol. 29, Issue 3, pp. 356-62, 2009 (PubMed).

Jursik, Prchal, Grillari-Voglauer et al.: "Large-scale production and characterization of novel CD4+ cytotoxic T cells with broad tumor specificity for immunotherapy." in: Molecular cancer research : MCR, Vol. 7, Issue 3, pp. 339-53, 2009 (PubMed).

Wu, Zhang, Zhang et al.: "Expansion of CD14+CD16+ peripheral monocytes among patients with aseptic loosening." in: Inflammation research : official journal of the European Histamine Research Society ... [et al.], Vol. 58, Issue 9, pp. 561-70, 2009 (PubMed).

Svensson: "Isolation and culture of human hematopoietic progenitors for studies of dendritic cell biology." in: Methods in molecular biology (Clifton, N.J.), Vol. 531, pp. 187-202, 2009 (PubMed).

Kuronuma, Mitsuzawa, Takeda et al.: "Anionic pulmonary surfactant phospholipids inhibit inflammatory responses from alveolar macrophages and U937 cells by binding the lipopolysaccharide-interacting proteins CD14 and MD-2." in: The Journal of biological chemistry, Vol. 284, Issue 38, pp. 25488-500, 2009 (PubMed).

Miko?ajczyk, Skrzeczy?ska-Moncznik, Zarebski et al.: "Interaction of human peripheral blood monocytes with apoptotic polymorphonuclear cells." in: Immunology, Vol. 128, Issue 1, pp. 103-13, 2009 (PubMed).

Schrauf, Kirchberger, Majdic et al.: "The ssRNA genome of human rhinovirus induces a type I IFN response but fails to induce maturation in human monocyte-derived dendritic cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 183, Issue 7, pp. 4440-8, 2009 (PubMed).

Schwartzkopff, Grimm, Lankford et al.: "Platelet factor 4 (CXCL4) facilitates human macrophage infection with HIV-1 and potentiates virus replication." in: Innate immunity, 2009 (PubMed).