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Lhca1 Antikörper

Lhca1 Reaktivität: Nicotiana tabacum, Oryza sativa, Pisum sativum, Arabidopsis thaliana, Spinach, Gerste, Mais (Zea mays), Green bean, Guzmania hybrid, Moss WB Wirt: Kaninchen Polyclonal unconjugated
Produktnummer ABIN5684014
  • Target
    Lhca1 (psi Type I Chlorophyll A/B-Binding Protein (Lhca1))
    Reaktivität
    Nicotiana tabacum, Oryza sativa, Pisum sativum, Arabidopsis thaliana, Spinach, Gerste, Mais (Zea mays), Green bean, Guzmania hybrid, Moss
    Wirt
    Kaninchen
    Klonalität
    Polyklonal
    Applikation
    Western Blotting (WB)
    Produktmerkmale
    Expected / apparent Molecular Weight of the Antigene: 22 / 22 kDa for Arabidopsis thaliana
    Aufreinigung
    affinity purified
    Immunogen
    BSA-conjugated synthetic peptide derived from the Lhca1 protein of Arabidopsis thaliana UniProt: Q01667, TAIR: At3g54890. This sequence is highly conserved in Lhca1 proteins of angiosperms (monocots and dicots) and gymnosperms.
    Isotyp
    IgG
  • Applikationshinweise
    Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).
    Kommentare

    A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA freepeptide, indicating that all bands are indeed PsbA and its precursors or breakdown product.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Lyophilized
    Rekonstitution
    For reconstitution add 200 µL of sterile water
    Buffer
    PBS pH 7.4
    Handhabung
    Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
    Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.
    Lagerung
    -20 °C
  • Busch, Petersen, Webber-Birungi, Powikrowska, Lassen, Naumann-Busch, Nielsen, Ye, Boekema, Jensen, Lunde, Jensen: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).

    Sakuraba, Rahman, Cho, Kim, Koh, Yoo, Paek: "The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 1, pp. 122-33, (2013) (PubMed).

    Aubry, Mani, Hoertensteiner: "Stay-green protein, defective in Mendel's green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway." in: Plant molecular biology, Vol. 67, Issue 3, pp. 243-56, (2008) (PubMed).

  • Target
    Lhca1 (psi Type I Chlorophyll A/B-Binding Protein (Lhca1))
    Hintergrund
    AGI Code: At3g54890
    The light-harvesting protein Lhca1 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca1 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca1 forms a heterodimer (LHCI-730) with Lhca4 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073
    Molekulargewicht
    expected: 22 kDa, apparent: 22 kDa for Arabidopsis thaliana
    UniProt
    Q01667
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