AKT3 Antikörper

Antigen: AKT3

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Maus, Ratte (Rattus)

Applikation: Western Blot (WB), ELISA, Immunpräzipitation (IP), Immunhistochemie (Paraffinschnitte) (IHC (p)), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)), Durchflusszytometrie (FACS), Immunfluoreszenz (IF)

Produktnummer: ABIN755527

Produktbeschreibung

Immunogen: Polyclonal antibodies are produced by immunizing animals with synthetic peptide derived from human AKT3

Format: Lyophilized

Wiederherstellung: Reconstitute with 100ul sterille distilled water. After reconstitution, stock concentration=1ug/1ul, preservatives included (BSA=10ug/ul, Sodium azide=0.1%)

Isotyp: IgG  (Passende Sekundärantikörper)

Beschreibung: Synonym: AKT3 kinase, DKFZP434N0250, PKB Gamma, PKBG, PRKBG, Protein Kinase AKT3, Protein Kinase B Gamma, RAC gamma, RAC Gamma Serine/Threonine Protein Kinase, RACPK Gamma, Serine threonine protein kinase Akt 3, Serine Threonine Protein Kinase AKT3, STK 2, STK2, V akt murine thymoma viral oncogene homolog 3 protein kinase B gamma, VAKT Murine Thymoma Viral Oncogene Homolog 3.

Molekulargewicht: 56kDa

Kommentare:

Akt

protein kinase B (PKB)

is a serine/threonine kinase which is involved in many cellular signaling pathways and acts as a transducer of many functions initiated by growth factor receptors that activate phosphtidylinositol 3 kinase (PI 3 kinase). The major activity of Akt/PKB is to mediate cell survival. Akt/PKB is also believed to be a critical factor in the genesis of cancer as the tumor suppressor PTEN was found to antagonise PI3 kinase and Akt/PKB kinase activity. There are 3 known isoforms of this enzyme in mammalian cells (1/alpha

2/beta and 3/gamma). IGF1 leads to the activation of AKT3

which may play a role in regulating cell survival. It is capable of phosphorylating several known proteins.

Anwendungen

Applikationshinweise: WB (1:200-600), ELISA (1:500-1000), IP (1:20-100), IHC-P (1:100-500), IHC-F (1:100-500), Flow-Cyt (1:20-100), IF (1:50-200)

Konzentration: 1mg/1ml

Reinigung: affinity purified by Protein A

Buffer: 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide

Lagerung: Ship at room temperature. Store at -20°C

Forschungsgebiet: Krebs

Beschränkungen: Nur für Forschungszwecke einsetzbar