FOXO1 Antikörper (pSer256)

Produktnummer ABIN498785

Der Kaninchen Polyklonal anti-FOXO1 Antikörper wird verwendet zum Nachweis von FOXO1 in Proben von Human, Maus und Ratte. Er wurde validiert für WB und IHC (p).

Kurzübersicht für FOXO1 Antikörper (pSer256) (ABIN498785)

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FOXO1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Produktdetails anti-FOXO1 Antikörper

Bindungsspezifität: pSer256

Spezifität: This antibody detects endogenous levels of p-FKHR/FOXO1A protein only when phosphorylated at Ser256 .

Kreuzreaktivität (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Aufreinigung: Affinity chromatography

Reinheit: > 95 % by SDS-PAGE

Anwendungsinformationen

Applikationshinweise: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Details zu FOXO1

Target: FOXO1 (Forkhead Box O1 (FOXO1))

Andere Bezeichnung: FOXO1A

Hintergrund: The transcription factor forkhead in rhabdomyosarcoma (FKHR), which is inhibited by insulin and IGF-1, enhances transcription. FKHR has been implicated in alveolar rhabdomyosarcoma, a soft tissue tumor wherein a chromosomal translocation [t(2,12)(q35,q14)] occurs between the FKHR and PAX3 genes, resulting in a novel chimeric protein with abnormal levels of expression. FKHR becomes phosphorylated at Ser 319, Ser 256 and Thr 256 by protein kinase B (PKB) in a phosphoinsoditide 3 (PI 3)-kinase/Akt-dependent pathway, resulting in the inactivation and subsequent nuclear exit of FKHR. In addition, FKHR becomes phosphorylated at Ser 329, also resulting in decreased FKHR activity and diminished nuclear FKHR concentration. However, phosphorylation of FKHR at Ser 329 is not mediated by a PI 3-kinasedependent pathway, but by an alternate mechanism. Dualspecificity tyrosine-phosphorylated and regulated kinase 1A (DYRK1A), which co-localizes to the same region of the nucleus as FKHR, specifically phosphorylates FKHR at Ser 329 in rabbit skeletal muscle.Synonyms: FKHR, FOXO1, Forkhead Box Protein O1, Forkhead Box Protein O1A, Forkhead in Rhabdomyosarcoma

Molekulargewicht: approx. 70 to 82 kDa

Gen-ID: 2308

NCBI Accession: NP_002006

UniProt: Q12778

Pathways: PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Carbohydrate Homeostasis, Chromatin Binding, Regulation of Carbohydrate Metabolic Process, CXCR4-mediated Signaling Events, BCR Signaling