NFIC Antikörper (AA 323-372)
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- Target Alle NFIC Antikörper anzeigen
- NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))
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Bindungsspezifität
- AA 323-372
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Reaktivität
- Human, Maus, Ratte, Hund, Rind (Kuh), Meerschweinchen, Schwein, Affe, Fledermaus
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Wirt
- Kaninchen
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Klonalität
- Polyklonal
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Konjugat
- Dieser NFIC Antikörper ist unkonjugiert
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Applikation
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Marke
- IHC-plus™
- Spezifität
- Human NFIC
- Homologie
- Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Bovine, Pig, Guinea pig (100%) Chicken (93%) Xenopus (86%).
- Aufreinigung
- Immunoaffinity purified
- Immunogen
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Synthetic peptide located between aa323-372 of human NFIC (Q6FI30, NP_995315). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Panda, Dog, Bovine, Bat, Pig, Guinea pig (100%), Zebra finch, Chicken (93%), Opossum (92%), Xenopus (86%), Stickleback (80%).
Type of Immunogen: Synthetic peptide - Isotyp
- IgG
- Top Product
- Discover our top product NFIC Primärantikörper
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- Applikationshinweise
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Approved: IHC, IHC-P (5 μg/mL), WB (1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL. Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:1562500. - Kommentare
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Target Species of Antibody: Human
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Format
- Lyophilized
- Rekonstitution
- Distilled water
- Konzentration
- Lot specific
- Buffer
- Lyophilized from PBS with 2 % sucrose
- Handhabung
- Aliquot to Avoid freeze/thaw cycles after reconstitution
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- Target
- NFIC (Nuclear Factor I/C (CCAAT-Binding Transcription Factor) (NFIC))
- Andere Bezeichnung
- NFI / NFIC (NFIC Produkte)
- Synonyme
- LOC100005568 antikoerper, 1110019L22Rik antikoerper, 1500041O16Rik antikoerper, AA589446 antikoerper, AI746521 antikoerper, NF1-C antikoerper, CTF antikoerper, CTF5 antikoerper, NF-I antikoerper, NFI antikoerper, CNFI-C antikoerper, nf1-c1 antikoerper, nuclear factor I/C antikoerper, nuclear factor I C antikoerper, nuclear factor I C L homeolog antikoerper, nfic antikoerper, NFIC antikoerper, Nfic antikoerper, nfic.L antikoerper
- Hintergrund
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Name/Gene ID: NFIC
Synonyms: NFIC, CTF, CTF5, NF1-C, NF-I/C, NFI, NFI-C, TGGCA-binding protein, NF-I, Nuclear factor 1 C-type, Nuclear factor 1/C, Nuclear factor I/C - Gen-ID
- 4782
- NCBI Accession
- NP_995315
- UniProt
- P08651
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