CBP Tag Antikörper

Details zu Produkt Nr. ABIN398464, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
14
1
Wirt
Kaninchen
9
6
Klonalität
Polyklonal
Applikation
ELISA, Western Blotting (WB)
15
5
2
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen A synthetic CBP tag peptide KRRWKKNFIAVSAANRFKKISSSGAL conjugated - KLH
Sequenz KRRWKKNFIA VSAA
Isotyp IgG
Spezifität CBP-tag Antibody recognizes either internal or C-terminal CBP tagged fusion proteins.
Reinigung Immunoaffinity chromatography
Andere Bezeichnung CBP-Tag
Substanzklasse Tag
Hintergrund Well-characterized antibodies consisting of short sequences have been widely and effectively used in the study of protein expression in various systems.CBP tag is a calmodulin-binding peptide, KRRWKKNFIAVSAANRFKKISSSGAL, derived from skeletal/cardiac muscle myosin light chain kinase 2.Rabbit Anti-CBP-tag Polyclonal Antibody is developed in rabbit hosts using a synthetic CBP tag peptide conjugated to KLH.CBP-tag Antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting CBP fusion proteins.
Applikationshinweise Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.

ELISA: 0.05-0.2 µg/mL Western blot:0.1-1.0 µg/mL Western Blot Using ONE-HOUR WesternTM Kit: For quick results, ONE-HOUR WesternTM Kit is recommendet. 10 µg of this antibody is mixed with 10 mL of WB solution for 8 cm x 8 cm membrane. Other applications: user-optimized
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Lyophilized
Buffer PBS, pH 7.4, containing 0.02 % Sodium azide
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-CBP Tag antibody (ABIN398464) Lane 1-2: CBP tag fusion protein expressed in E. coli Cell Lysate Antibody: 1 µg/mL R...
Western Blotting (WB) image for anti-CBP Tag antibody (ABIN398464) Western blot analysis of CBP tagged fusion proteins expressed in E. coli cell lysate ...
Produkt verwendet in: Yang, Wang, Ju, Ragab, Lundbäck, Long, Valdes-Ferrer, He, Pribis, Li, Lu, Gero, Szabo, Antoine, Harris, Golenbock, Meng, Roth, Chavan, Andersson, Billiar, Tracey, Al-Abed: "MD-2 is required for disulfide HMGB1-dependent TLR4 signaling." in: The Journal of experimental medicine, Vol. 212, Issue 1, pp. 5-14, 2015 (PubMed).

Park, Ménétret, Gumbart, Ludtke, Li, Whynot, Rapoport, Akey: "Structure of the SecY channel during initiation of protein translocation." in: Nature, Vol. 506, Issue 7486, pp. 102-6, 2014 (PubMed).

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Wanschers, Szklarczyk, Pajak, van den Brand, Gloerich, Rodenburg, Lightowlers, Nijtmans, Huynen: "C7orf30 specifically associates with the large subunit of the mitochondrial ribosome and is involved in translation." in: Nucleic acids research, Vol. 40, Issue 9, pp. 4040-51, 2012 (PubMed).

Carnes, Schnaufer, McDermott, Domingo, Proff, Steinberg, Kurtz, Stuart: "Mutational analysis of Trypanosoma brucei editosome proteins KREPB4 and KREPB5 reveals domains critical for function." in: RNA (New York, N.Y.), Vol. 18, Issue 10, pp. 1897-909, 2012 (PubMed).

Woods, Mesquita, Sweet, Carvalho, Li, Liu, Nguyen, Thomas, Iversen, Marsillac, Karchin, Koomen, Monteiro: "Charting the landscape of tandem BRCT domain-mediated protein interactions." in: Science signaling, Vol. 5, Issue 242, pp. rs6, 2012 (PubMed).

Ringpis, Lathrop, Aphasizhev: "iCODA: RNAi-based inducible knock-in system in Trypanosoma brucei." in: Methods in molecular biology (Clifton, N.J.), Vol. 718, pp. 23-37, 2011 (PubMed).

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Shin, Wolgamott, Yu, Blenis, Yoon: "Glycogen synthase kinase (GSK)-3 promotes p70 ribosomal protein S6 kinase (p70S6K) activity and cell proliferation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 47, pp. E1204-13, 2011 (PubMed).

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