p21 Antikörper (AA 143-147)

Produktnummer ABIN362754

Zitiert in 1 Publikation. Der Kaninchen Polyklonal anti-p21 Antikörper (ABIN362754) detektiert spezifisch p21 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Human.

Kurzübersicht für p21 Antikörper (AA 143-147) (ABIN362754)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser p21 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-p21 Antikörper

Bindungsspezifität: AA 143-147

Spezifität: The antibody detects endogenous level of total p21Cip1 protein.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Peptide sequence around AA 143-147 (R-Q-T-S-M) derived from Human p21Cip1. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-p21 Antikörper (ABIN362754)

Kanehira, Kikuchi, Ohkouchi, Shibahara, Tode, Santoso, Daito, Ohta, Tamada, Nukiwa: "Targeting lysophosphatidic acid signaling retards culture-associated senescence of human marrow stromal cells." in: PLoS ONE, Vol. 7, Issue 2, pp. e32185, (2012) (PubMed).

Details zu p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Andere Bezeichnung: p21Cip1

Hintergrund: May be the important intermediate by which p53 mediates its role as an inhibitor of cellular proliferation in response to DNA damage. Binds to and inhibits cyclin-dependent kinase activity, preventing phosphorylation of critical cyclin-dependent kinase substrates and blocking cell cycle progression.

Molekulargewicht: 21 kDa

UniProt: P38936

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, AMPK Signaling, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagie