STAT1 Antikörper (pTyr701)

Produktnummer ABIN361957

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch STAT1 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte. Es wurde in 3+ Publikationen zitiert.

Kurzübersicht für STAT1 Antikörper (pTyr701) (ABIN361957)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-STAT1 Antikörper

Bindungsspezifität: pTyr701

Spezifität: The antibody detects endogenous level of STAT1 only when phosphorylated at tyrosine 701.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pTyr701 (T-G-Y (p) -I-K) derived from Human STAT1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-STAT1 Antikörper (ABIN361957)

Hara, Nakamura, Ninomiya, Mochizuki, Kamiya, Aizenman, Koketsu, Adachi: "Inhibitory effects of chalcone glycosides isolated from Brassica rapa L. 'hidabeni' and their synthetic derivatives on LPS-induced NO production in microglia." in: Bioorganic & medicinal chemistry, Vol. 19, Issue 18, pp. 5559-68, (2011) (PubMed).

Fros, Liu, Prow, Geertsema, Ligtenberg, Vanlandingham, Schnettler, Vlak, Suhrbier, Khromykh, Pijlman: "Chikungunya virus nonstructural protein 2 inhibits type I/II interferon-stimulated JAK-STAT signaling." in: Journal of virology, Vol. 84, Issue 20, pp. 10877-87, (2010) (PubMed).

Chen, Ma, Sytwu, Wang, Chen, Liu, Chen, Chen, Wang: "Neural stem cells secrete factors that promote auditory cell proliferation via a leukemia inhibitory factor signaling pathway." in: Journal of neuroscience research, Vol. 88, Issue 15, pp. 3308-18, (2010) (PubMed).

Details zu STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Andere Bezeichnung: STAT1

Hintergrund: Signal transducer and activator of transcription that mediates signaling by interferons (IFNs). Following type I IFN (IFN-a and IFN-beta) binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize, associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated. It then forms a homodimer termed IFN-gamma-activated factor (GAF), migrates into the nucleus and binds to the IFN gamma activated sequence (GAS) to drive the expression of the target genes, inducing a cellular antiviral state.

Molekulargewicht: 91 kDa

UniProt: P42224

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events