GSK3 alpha Antikörper (pSer21)

Produktnummer ABIN361885

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GSK3 alpha in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.Es wurde in 1 Publikation zitiert.

Kurzübersicht für GSK3 alpha Antikörper (pSer21) (ABIN361885)

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GSK3 alpha Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-GSK3 alpha Antikörper

Bindungsspezifität: pSer21

Spezifität: The antibody detects endogenous level of GSK3a only when phosphorylated at serine 21and does not detect GSK-3b when phosphorylated at Ser9.

Aufreinigung: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer21 (T-S-S (p) -F-A) derived from Human GSK3a. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Referenzen für anti-GSK3 alpha Antikörper (ABIN361885)

Liu, Huang, Zeng, Chen, Huang, Guo, Liu, Xu, Mo, Li: "Down-regulation of JAK1 by RNA interference inhibits growth of the lung cancer cell line A549 and interferes with the PI3K/mTOR pathway." in: Journal of cancer research and clinical oncology, Vol. 137, Issue 11, pp. 1629-40, (2011) (PubMed).

Details zu GSK3 alpha

Target: GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Andere Bezeichnung: GSK3alpha

Hintergrund: Implicated in the hormonal control of several regulatory proteins including glycogen synthase,

Molekulargewicht: 51 kDa

NCBI Accession: NP_063937

UniProt: P49840

Pathways: PI3K-Akt Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effekt