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HSP70/HSC70 Antikörper

HSC70-4 Reaktivität: Huhn WB, IP, IHC, ICC, IF Wirt: Maus Monoclonal BB70 unconjugated
Produktnummer ABIN361709
  • Target Alle HSP70/HSC70 (HSC70-4) Produkte
    HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))
    Reaktivität
    • 34
    • 34
    • 25
    • 23
    • 23
    • 23
    • 23
    • 22
    • 22
    • 21
    • 21
    • 21
    • 21
    • 20
    • 20
    • 20
    • 12
    • 11
    • 10
    • 10
    • 10
    • 4
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Huhn
    Wirt
    • 53
    • 2
    Maus
    Klonalität
    • 53
    • 2
    Monoklonal
    Konjugat
    • 10
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    Dieser HSP70/HSC70 Antikörper ist unkonjugiert
    Applikation
    • 55
    • 42
    • 41
    • 22
    • 20
    • 19
    • 19
    • 10
    • 10
    • 2
    Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)
    Spezifität
    Detects ~72 (HSP) and ~73 kDa (HSC).
    Kreuzreaktivität
    Beluga, Huhn, Rind (Kuh), Hund, Drosophila melanogaster, Fisch, Meerschweinchen, Hamster, Human, Maus, Schwein, Kaninchen, Ratte, Saccharomyces cerevisiae, Schaf, Xenopus laevis
    Aufreinigung
    Protein G Purified
    Immunogen
    Chicken HSP70/HSP90 complex
    Klon
    BB70
    Isotyp
    IgG2a
  • Applikationshinweise
    • WB (1:1000)
    • IHC (1:200)
    • ICC/IF (1:200)
    • optimal dilutions for assays should be determined by the user.
    Kommentare

    1 μg/ml of ABIN361709 was sufficient for detection of HSP70 and HSC70 in 20 μg of heat shocked HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody.

    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Format
    Liquid
    Konzentration
    1 mg/mL
    Buffer
    PBS pH 7.2, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated
    Konservierungsmittel
    Sodium azide
    Vorsichtsmaßnahmen
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Lagerung
    -20 °C
    Informationen zur Lagerung
    -20°C
  • Kang, Taldone, Patel, Patel, Rodina, Gozman, Maharaj, Clement, Patel, Brodsky, Young, Chiosis: "Heat shock protein 70 inhibitors. 1. 2,5'-thiodipyrimidine and 5-(phenylthio)pyrimidine acrylamides as irreversible binders to an allosteric site on heat shock protein 70." in: Journal of medicinal chemistry, Vol. 57, Issue 4, pp. 1188-207, (2014) (PubMed).

    Taldone, Kang, Patel, Patel, Patel, Rodina, Patel, Gozman, Maharaj, Clement, Lu, Young, Chiosis et al.: "Heat shock protein 70 inhibitors. 2. 2,5'-thiodipyrimidines, 5-(phenylthio)pyrimidines, 2-(pyridin-3-ylthio)pyrimidines, and 3-(phenylthio)pyridines as reversible binders to an allosteric site on ..." in: Journal of medicinal chemistry, Vol. 57, Issue 4, pp. 1208-24, (2014) (PubMed).

    Rodina, Taldone, Kang, Patel, Koren, Yan, DaGama Gomes, Yang, Patel, Shrestha, Ochiana, Santarossa, Maharaj, Gozman, Cox, Erdjument-Bromage, Hendrickson, Cerchietti, Melnick, Guzman, Chiosis: "Affinity purification probes of potential use to investigate the endogenous Hsp70 interactome in cancer." in: ACS chemical biology, Vol. 9, Issue 8, pp. 1698-705, (2014) (PubMed).

    Rodina, Patel, Kang, Patel, Baaklini, Wong, Taldone, Yan, Yang, Maharaj, Gozman, Patel, Patel, Chirico, Erdjument-Bromage, Talele, Young, Chiosis: "Identification of an allosteric pocket on human hsp70 reveals a mode of inhibition of this therapeutically important protein." in: Chemistry & biology, Vol. 20, Issue 12, pp. 1469-80, (2013) (PubMed).

  • Target
    HSP70/HSC70 (HSC70-4) (Heat Shock Protein Cognate 4 (HSC70-4))
    Andere Bezeichnung
    HSP70/HSC70 (HSC70-4 Produkte)
    Synonyme
    BAP74 antikoerper, CG4264 antikoerper, Dmel\\CG4264 antikoerper, E(csp)1545 antikoerper, E(nd)195 antikoerper, HSC-70 antikoerper, HSC4 antikoerper, HSC70 antikoerper, HSC70-4 antikoerper, Hsc-4 antikoerper, Hsc4 antikoerper, Hsc4p antikoerper, Hsc70 antikoerper, Hsp-c4 antikoerper, Hsp70 antikoerper, anon-WO0118547.237 antikoerper, bs17d06.y1 antikoerper, dhsc70 antikoerper, hsc4 antikoerper, hsc70 antikoerper, hsc70-4 antikoerper, hsp70 antikoerper, i190 antikoerper, l(3)03550 antikoerper, l(3)L3929 antikoerper, l(3)j7A4 antikoerper, scd antikoerper, Heat shock protein cognate 4 antikoerper, Hsc70-4 antikoerper
    Hintergrund
    HSP70 genes encode abundant heat-inducible 70- kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50 % identity (2). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (3). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (4). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (5). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (6). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
    Gen-ID
    423504
    NCBI Accession
    NP_001006686
    UniProt
    P08106
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