Protein C Tag (NULL) Antikörper

Details zu Produkt Nr. ABIN356540, Anbieter: Anmelden zum Anzeigen
Antigen
Reaktivität
Tag
4
Wirt
Kaninchen
3
1
Klonalität
Polyklonal
Applikation
Enzyme Immunoassay (EIA), Western Blotting (WB)
4
2
1
1
1
1
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen KLH conjugated synthetic peptide encoding the Protein-C epitope tag (CEDQVDPRLIDGK) was used as antigen.
Isotyp Ig
Spezifität This antibody is specific to Protein-C Tag.
Reinigung Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Substanzklasse Tag
Hintergrund Epitope tags consisting of short sequences recognized by well-characterizated antibodies have been widely used in the study of protein expression in various systems. The epitope tag composed of a 12 residue peptide, EDQVDPRLIDGK , is derived from Protein-C, a vitamin K-dependent serine protease that regulates blood coagluation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids. This polyclonal antibody provides a simple solution to detect the expression of a Protein-C epitope-tagged protein in cells.Synonyms: C tag, C-Tag, Protein C Epitope tag
Gen-ID 5624, 5874
UniProt P04070
Applikationshinweise ELISA: 1/5,000. Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration 0.25 mg/mL
Buffer PBS containing 0.09 % (W/V) Sodium Azide as preservative.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freezing and thawing.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
Bilder des Herstellers
 image for anti-Protein C Tag (NULL) antibody (ABIN356540) anti-Protein C Tag (NULL) antibody
Allgemeine Veröffentlichungen Lichty, Malecki, Agnew, Michelson-Horowitz, Tan: "Comparison of affinity tags for protein purification." in: Protein expression and purification, Vol. 41, Issue 1, pp. 98-105, 2005 (PubMed).

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