Maus anti-Human IgG (Fab Region) Antikörper (PE)

Details zu Produkt Nr. ABIN349709, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Fab Region
4681
2537
1879
1066
1006
860
297
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Human
2849
2735
2296
1675
1433
788
613
442
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Wirt
Maus
6705
4831
1795
890
609
388
91
55
52
21
8
5
5
5
2
1
1
Klonalität (Klon)
Monoklonal ()
Konjugat
PE
2236
1872
1738
1356
767
682
519
296
264
223
222
200
156
138
128
110
79
75
69
55
51
46
43
40
38
36
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
12
10
10
9
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Flow Cytometry (FACS)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Purified human IgG.
Klon 4A11
Isotyp IgG2b
Spezifität The antibody 4A11 reacts with Fab fragment of human IgG.
Produktmerkmale The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography and adjusted for direct use.
Hintergrund Immunoglobulin G (IgG) is a 150 kDa soluble protein that serves as a major effector molecule of the humoral immune response in man. Its concentration in blood plasma of healthy individuals is approximately 10 g/l, which accounts for about 75 % of the total plasma immunoglobulins. IgG has the highest stability of blood immunoglobulins (T1/2 = 21 days) and is able of placental transfer. IgG is secreted by plasma cells at a comparably high rate as other immunoglobulins.
Molekulargewicht 150 kDa
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise The reagent is designed for Flow Cytometry analysis. Suggested working dilution is 6 µg/mL. Indicated dilution is recommended starting point for use of this product. Working concentrations should be determined by the investigator.

Working concentrations should be determined by the investigator.
Kommentare

Excitation/Emission wavelength: 496 nm/576 nm

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Rekonstitution No reconstitution is necessary.
Konzentration 0.1 mg/mL
Buffer The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2 % (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
Handhabung Do not freeze.
Avoid prolonged exposure to light.
Lagerung 4 °C
Informationen zur Lagerung Store in the dark at 2-8 °C. Do not use after expiration date stamped on vial label. Short-term exposure to room temperature should not affect the quality of the reagent. However, if reagent is stored under any conditions other than those specified, the conditions must be verified by the user.
Bilder des Herstellers
Western Blotting (WB) image for Mouse anti-Human IgG (Fab Region) antibody (PE) (ABIN349709) Western Blotting analysis (reducing conditions) of human IgG Fab fragment using anti-...
Allgemeine Veröffentlichungen Brinkmann, Heusser: "T cell-dependent differentiation of human B cells into IgM, IgG, IgA, or IgE plasma cells: high rate of antibody production by IgE plasma cells, but limited clonal expansion of IgE precursors." in: Cellular immunology, Vol. 152, Issue 2, pp. 323-32, 1994 (PubMed).

Balogh, Merétey, Falus, Bozsóky: "Serological abnormalities in juvenile chronic arthritis: a review of 46 cases." in: Annals of the rheumatic diseases, Vol. 39, Issue 2, pp. 129-34, 1980 (PubMed).

Fuller, Keyser: "Serum immunoglobulins after surgical operation." in: Clinical chemistry, Vol. 21, Issue 6, pp. 667-71, 1975 (PubMed).

Franklin: "Structure and function of immunoglobulins." in: Acta endocrinologica. Supplementum, Vol. 194, pp. 77-95, 1975 (PubMed).