CD14 Molecule (CD14) Antikörper

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Funktionale Studien (Func), Immunpräzipitation (IP)

Produktnummer: ABIN343181

Produktbeschreibung

Weitere Bezeichnung: CD14

Immunogen: Affinity purified recombinant human CD14

Format: Lyophilized

Beschreibung: The antiserum represents a marker for human CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Anwendungen

Applikationshinweise: Suitable for Immunostaining of CD14 positive cells, Immunoprecipitation, Western blot. no significant but small inhibition of LPS binding to human CD14, Binding titre at human CD14+CHO cells (FACS) more than 1:5.000. Recommended working dilution: 1: 100 till 1.000 Binding at mouse CD14 transfected CHO+ cells more than 1:1000 (recommanded working dilution 1:10 til 1:100)

Reinigung: Protein G purified

Buffer: 0.1 ml without NaN3

Lagerung: long time storage at -20 °C or

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Baveye, Elass, Fernig et al.: "Human lactoferrin interacts with soluble CD14 and inhibits expression of endothelial adhesion molecules, E-selectin and ICAM-1, induced by the CD14-lipopolysaccharide complex." in: Infection and immunity, Vol. 68, Issue 12, pp. 6519-25, 2000 (PubMed).

Vanlandschoot, Van Houtte, Roobrouck et al.: "LPS-binding protein and CD14-dependent attachment of hepatitis B surface antigen to monocytes is determined by the phospholipid moiety of the particles." in: The Journal of general virology, Vol. 83, Issue Pt 9, pp. 2279-89, 2002 (PubMed).