CD14 Molecule (CD14) Antikörper

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (BiG 7)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), Funktionale Studien (Func), ELISA

Produktnummer: ABIN343172

Produktbeschreibung

Weitere Bezeichnung: CD14

Immunogen: Monocytes and immunoaffinity purified soluble CD14

Format: Lyophilized

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: BiG 7

Beschreibung: BiG 7 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Anwendungen

Applikationshinweise: Suitable for immunostaining of CD14 positive cells, (Flow cytometry), ELISA, Suitable for Western blot (non reducing). Non inhibition of LPS binding to CD14, binding epitope: amino acid 9-13, 39-44, Binding titre: 1:10.000

Konzentration: 1 mg/ml

Reinigung: Protein G purified

Buffer: 0.1 mg/100 ul PBS without NaN3

Lagerung: long time storage at -20C or

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Stelter, Bernheiden, Menzel et al.: "Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli." in: European journal of biochemistry / FEBS, Vol. 243, Issue 01. Feb, pp. 100-9, 1997 (PubMed).

Dziarski, Tapping, Tobias: "Binding of bacterial peptidoglycan to CD14." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8680-90, 1998 (PubMed).

Fan, Stelter, Menzel et al.: "Structures in Bacillus subtilis are recognized by CD14 in a lipopolysaccharide binding protein-dependent reaction." in: Infection and immunity, Vol. 67, Issue 6, pp. 2964-8, 1999 (PubMed).

Neilsen, Zimmerman, McIntyre: "Escherichia coli Braun lipoprotein induces a lipopolysaccharide-like endotoxic response from primary human endothelial cells." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 167, Issue 9, pp. 5231-9, 2001 (PubMed).

Muansson, Kjaell, Pellett et al.: "Role of the lipopolysaccharide-CD14 complex for the activity of hemolysin from uropathogenic Escherichia coli." in: Infection and immunity, Vol. 75, Issue 2, pp. 997-1004, 2007 (PubMed).