CD14 Molecule (CD14) Antikörper

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (RoMo-1)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Funktionale Studien (Func), ELISA

Produktnummer: ABIN343170

Produktbeschreibung

Weitere Bezeichnung: CD14

Immunogen: Human Monocytes

Format: Lyophilized

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: RoMo-1

Beschreibung: BiG 2 (RoMo-1) represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Anwendungen

Applikationshinweise: Suitable for Immunostaining of CD14 positive cells (Flow cytometry), Immunoprecipitation, ELISA. Not useful for Western blot. No significant inhibition of LPS binding to CD14, binding epitope amino acid147-152 Binding titre at CD14+CHO cells (FACS) 1:5.000

Konzentration: 1 mg/ml

Reinigung: Protein G purified

Buffer: 0.1 mg in PBS without NaN3

Lagerung: long time storage at -20C or

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Stelter, Bernheiden, Menzel et al.: "Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli." in: European journal of biochemistry / FEBS, Vol. 243, Issue 01. Feb, pp. 100-9, 1997 (PubMed).

Dziarski, Tapping, Tobias: "Binding of bacterial peptidoglycan to CD14." in: The Journal of biological chemistry, Vol. 273, Issue 15, pp. 8680-90, 1998 (PubMed).

Neumeister, Faigle, Sommer et al.: "Low endotoxic potential of Legionella pneumophila lipopolysaccharide due to failure of interaction with the monocyte lipopolysaccharide receptor CD14." in: Infection and immunity, Vol. 66, Issue 9, pp. 4151-7, 1998 (PubMed).

Stelter, Loppnow, Menzel et al.: "Differential impact of substitution of amino acids 9-13 and 91-101 of human CD14 on soluble CD14-dependent activation of cells by lipopolysaccharide." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 163, Issue 11, pp. 6035-44, 1999 (PubMed).

Vanlandschoot, Van Houtte, Roobrouck et al.: "LPS-binding protein and CD14-dependent attachment of hepatitis B surface antigen to monocytes is determined by the phospholipid moiety of the particles." in: The Journal of general virology, Vol. 83, Issue Pt 9, pp. 2279-89, 2002 (PubMed).