CD14 Molecule (CD14) Antikörper

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (BiG 14)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), Funktionale Studien (Func), ELISA

Produktnummer: ABIN343169

Produktbeschreibung

Weitere Bezeichnung: CD14

Immunogen: Monocytes of different species and immunoaffinity purified soluble human CD14

Format: Lyophilized

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: BiG 14

Beschreibung: BiG 14 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxin.

Anwendungen

Applikationshinweise: Suitable for immunostaining of CD14 positive cells (Flow cytometry), ELISA, CD14 inhibition studies, Western Blot. 1 ug/ml inhibits binding of 0.5 ug/ ml LPS to CD14, binding epitope amino acid 39-44, binding epitope aa 39-41, 43-44 Cross reacting with CD14 of cattle++, swine ++, dog+ horse+, guinea pig +, Inhibits binding of LPS to CD14 of swine++, dog+, cattle +, dog+ Binding titre at human CD14 transfected CHO-cells: more than 10.000 Stimulated LPS induced TNF and IL1-secretion.

Konzentration: 1 mg/ml

Reinigung: Protein G purified

Buffer: 0.1mg in PBS without NaN3

Lagerung: long time storage at -20C or

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Stelter, Bernheiden, Menzel et al.: "Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli." in: European journal of biochemistry / FEBS, Vol. 243, Issue 01. Feb, pp. 100-9, 1997 (PubMed).

Bruzzone, De Flora, Usai et al.: "Cyclic ADP-ribose is a second messenger in the lipopolysaccharide-stimulated proliferation of human peripheral blood mononuclear cells." in: The Biochemical journal, Vol. 375, Issue Pt 2, pp. 395-403, 2003 (PubMed).

Schromm, Reiling, Howe et al.: "Influence of serum on the immune recognition of a synthetic lipopeptide mimetic of the 19-kDa lipoprotein from Mycobacterium tuberculosis." in: Innate immunity, 2009 (PubMed).

Bache, Spreitzer, Becker et al.: "Bordetella Pertussis Toxin does not induce the release of pro-inflammatory cytokines in human whole blood." in: Medical microbiology and immunology, 2012 (PubMed).