CD14 Molecule (CD14) Antikörper

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (BiG 10)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Western Blot (WB), Funktionale Studien (Func), ELISA

Produktnummer: ABIN343167

Produktbeschreibung

Weitere Bezeichnung: CD14

Immunogen: Monocytes and immunoaffinity purified soluble CD14

Format: Lyophilized

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: BiG 10

Beschreibung: BiG 10 represents an excellent marker for CD14. The CD14 glycoprotein, gp 55, is present on most monocytic and macrophages like cell types: monocytes, macrophages, Kupffer cells, pleural phagocytic cells and dendritic reticular cells. CD14 is also observed on granulocytes and activated or transformed B-cells. Furthermore CD14 is present in a soluble form in human serum, urine and other body fluids. The CD14 molecule has been reported to be a receptor for endotoxi.

Anwendungen

Applikationshinweise: Suitable for immunostaining of CD14positive cells (Flow cytometry), ELISA, CD14 inhibition studies, Western Blot. Inhibits binding of LPS to CD14, binding epitope: amino acid 9-13, 39-44, and total inhibition of LPS-binding to CD14: 100ug/ml Binding titre at human CD14 transfected CHO-cells: more than 10.000. It cross reacts with horse and bovine CD14 and inhibits binding of LPS to horse and bovine CD14. Binding titre at horse CD14 1:100 and inhibition titre 1:10.

Konzentration: 1 mg/ml

Reinigung: Protein G purified

Buffer: 100 ug/100 ul PBS without NaN3 before lyophilization

Lagerung: Long time storage at

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Stelter, Pfister, Bernheiden et al.: "The myeloid differentiation antigen CD14 is N- and O-glycosylated. Contribution of N-linked glycosylation to different soluble CD14 isoforms." in: European journal of biochemistry / FEBS, Vol. 236, Issue 2, pp. 457-64, 1996 (PubMed).

Stelter, Bernheiden, Menzel et al.: "Mutation of amino acids 39-44 of human CD14 abrogates binding of lipopolysaccharide and Escherichia coli." in: European journal of biochemistry / FEBS, Vol. 243, Issue 01. Feb, pp. 100-9, 1997 (PubMed).

Steinbach, Bischoff, Freund et al.: "Clinical application of dendritic cells and interleukin-2 and tools to study activated T cells in horses--first results and implications for quality control." in: Veterinary immunology and immunopathology, Vol. 128, Issue 01. Mrz, pp. 16-23, 2009 (PubMed).

Muerant, Breathnach, Kohler et al.: "Young foal and adult horse monocyte-derived dendritic cells differ by their degree of phenotypic maturity." in: Veterinary immunology and immunopathology, 2009 (PubMed).

Hackett, Flaminio, Fortier: "Analysis of CD14 Expression Levels in Putative Mesenchymal Progenitor Cells Isolated from Equine Bone Marrow." in: Stem cells and development, 2010 (PubMed).