TOP2 Antikörper (C-Term, N-Term)

Produktnummer ABIN334538

Produktdetails anti-TOP2 Antikörper

Target: TOP2 (DNA Topoisomerase II (TOP2))

Bindungsspezifität: C-Term, N-Term

Reaktivität: Arabidopsis thaliana, Vicia faba

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser TOP2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunolocalization (IL)

Spezifität: Antibody detects a protein of ca. 170 kDa on western blots of Arabidopsis thaliana protein extracts. In subcellular fractions of cultured Arabidopsis cells the antibody detects a 170 kDa protein exclusively in the nulear fraction.

Kreuzreaktivität (Details): Not reactive in: Nicotiana tabacum

Homologie: Brassica rapa, Chlamydomonas reinhardtii, Chlorella vulgaris, Citrus clementina, Glycine max, Hordeum vulgare, Medicago truncatula, Oryza sativa, Ostreococcus tauri, Panicum italicum, Phaseolus vulgaris, Physcomitrella patens, Pinus sitchensis, Populus trichocarpa, Solanum tuberosum, Sorghum bicolor, Triticum aestivum, Vitis vinifera, Volvox caterii

Produktmerkmale: Expected / apparent Molecular Weight of the Antigene: 164 / 170 kDa (Arabidopsis thaliana)

Aufreinigung: serum

Immunogen: The C-terminal 153 amino acids of the Arabidopsis thaliana Topoisomerase II (At3g23890, protein accesion number P30182) with an N-terminal hexahistidine tag was expressed in E.coli and purified by Ni2+ affinity chromatography.

Anwendungsinformationen

Applikationshinweise: 1: 2000 (WB), 1: 500 (IL)

Kommentare:

Topoisomerase II is highly expressed in young seedlings. The protein is localized in the nucleus and gene expression levels are increased in proliferative tissues like shoot apex or root tip.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: For reconstitution add 200 μL of sterile water

Handhabung: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Referenzen für anti-TOP2 Antikörper (ABIN334538)

Xie, Lam: "Characterization of a DNA Topoisomerase II cDNA from Arabidopsis thaliana." in: Plant physiology, Vol. 106, Issue 4, pp. 1701-2, (1995) (PubMed).

Details zu TOP2

Target: TOP2 (DNA Topoisomerase II (TOP2))

Andere Bezeichnung: TOP2 (TOP2 Produkte)

Synonyme: Topoii antikoerper, ptr11 antikoerper, DDBDRAFT_0191045 antikoerper, DDBDRAFT_0231510 antikoerper, DDB_0191045 antikoerper, DDB_0231510 antikoerper, CG10223 antikoerper, CHRAC antikoerper, Dmel\\CG10223 antikoerper, TOP2 antikoerper, TOPO II antikoerper, TOPOII antikoerper, TopII antikoerper, Topo II antikoerper, TopoII antikoerper, dCHRAC antikoerper, dTopoII antikoerper, top2 antikoerper, topo II antikoerper, topoII antikoerper, topoisomerase II antikoerper, DNA topoisomerase II antikoerper, DNA topoisomerase 2 antikoerper, DNA topoisomerase ii antikoerper, type IIA topoisomerase; DNA gyrase topoisomerase IV B subunit antikoerper, Topoisomerase 2 antikoerper, TOP2 antikoerper, A583L antikoerper, top2 antikoerper, CNA03600 antikoerper, Tb09.160.4090 antikoerper, Tb11.01.3390 antikoerper, LMJF_28_2280 antikoerper, Top2 antikoerper

Hintergrund: AGI Code: At3g23890
Topoisomerase type II (EC5.99.1.3) is one of the enzymes which is catalyzing unknotting of DNA by creating transient breaks in the DNA using a conserved tyrosine as the catalytic residue.Synonyme names of this protein: At3g23890, ATTOPII, DNA topoisomerase 2, DNA topoisomerase II, F14O13.7, TOP2, TOPOISOMERASE II

Molekulargewicht: expected: 164 kDa, apparent: 170 kDa (Arabidopsis thaliana)

UniProt: P30182

Pathways: Mitotic G1-G1/S Phases