SIRT2 Antikörper (Middle Region)

Produktnummer ABIN3043924

Produktdetails anti-SIRT2 Antikörper

Target: SIRT2 (Sirtuin 2 (SIRT2))

Bindungsspezifität: AA 165-187, Middle Region

Reaktivität: Human, Ratte, Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser SIRT2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Verwendungszweck: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-2(SIRT2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.

Sequenz: YTQNIDTLER IAGLEQEDLV EAH

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for NAD-dependent protein deacetylase sirtuin-2(SIRT2) detection. Tested with WB, IHC-P in Human,Mouse,Rat.
Gene Name: sirtuin 2
Protein Name: NAD-dependent protein deacetylase sirtuin-2

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human SIRT2(165-187aa YTQNIDTLERIAGLEQEDLVEAH), different from the related mouse and rat sequences by three amino acids.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, The detection limit for SIRT2 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Mouse, Rat, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Details zu SIRT2

Target: SIRT2 (Sirtuin 2 (SIRT2))

Andere Bezeichnung: SIRT2 (SIRT2 Produkte)

Synonyme: CG5085 antikoerper, D.mel2 antikoerper, Dmel\\CG5085 antikoerper, SIRT2 antikoerper, dSIRT2 antikoerper, sir2 antikoerper, Sirt2 antikoerper, BmSirt2 antikoerper, AtSRT2 antikoerper, SIRTUIN 2 antikoerper, T2K12.8 antikoerper, sirtuin 2 antikoerper, SIR2 antikoerper, SIR2L antikoerper, SIR2L2 antikoerper, 5730427M03Rik antikoerper, Sir2l antikoerper, wu:fb57d05 antikoerper, zgc:55966 antikoerper, zgc:77003 antikoerper, Sirtuin 2 antikoerper, sirtuin 2 antikoerper, sirtuin 2 S homeolog antikoerper, NAD-dependent deacetylase sirtuin 2 homolog antikoerper, sirtuin 2 (silent mating type information regulation 2, homolog) 2 (S. cerevisiae) antikoerper, Sirt2 antikoerper, SIRT2 antikoerper, sirt2.S antikoerper, SRT2 antikoerper, sirt2 antikoerper

Hintergrund: SIRT2 is a predominantly cytoplasmic protein that colocalizes with microtubules. This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. SIRT2 is mapped to 19q13.2. It is able to cause radioactivity to be transferred from (32P) NAD to bovine serum albumin (BSA). According to researches, SIRT2 can act as a bona fide tubulin deacetylase. SIRT2 was found to deacetylate lys40 of alpha-tubulin both in vitro and in vivo. SIRT2 is also a regulator of mitotic progression that acts downstream from CDC14B in a pathway regulating mitotic exit or subsequent cytokinesis. What's more, SIRT2 is an important regulator of programmed necrosis.

Synonyms: FLJ35621 antibody|FLJ37491 antibody|NAD dependent deacetylase sirtuin 2 antibody|NAD-dependent deacetylase sirtuin-2 antibody|NAD-dependent protein deacetylase sirtuin-2 antibody|Regulatory protein SIR2 homolog 2 antibody|Silencing information regulator 2 like antibody|Silent information regulator 2 antibody|Silent mating type information regulation 2 antibody|Silent mating type information regulation 2 homolog antibody|SIR 2 antibody|SIR2 antibody|SIR2 like antibody|SIR2 like protein 2 antibody|Sir2 related protein type 2 antibody|SIR2, S. cerevisiae, homolog-loke 2 antibody|SIR2-like protein 2 antibody|SIR2L antibody|SIR2L2 antibody|SIRT 2 antibody|SIRT2 antibody|SIRT2_HUMAN antibody|Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae) antibody|Sirtuin 2 antibody|Sirtuin type 2 antibody|Sirtuin2 antibody

Gen-ID: 22933