POU2F1 Antikörper (AA 11-240)
-
- Target Alle POU2F1 Antikörper anzeigen
- POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))
-
Bindungsspezifität
- AA 11-240
-
Reaktivität
- Human
-
Wirt
- Kaninchen
-
Klonalität
- Polyklonal
-
Konjugat
- Dieser POU2F1 Antikörper ist unkonjugiert
-
Applikation
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Verwendungszweck
- Rabbit IgG polyclonal antibody for POU domain, class 2, transcription factor 1(POU2F1) detection. Tested with WB, IHC-P in Human.
- Kreuzreaktivität (Details)
- No cross reactivity with other proteins.
- Produktmerkmale
-
Rabbit IgG polyclonal antibody for POU domain, class 2, transcription factor 1(POU2F1) detection. Tested with WB, IHC-P in Human.
Gene Name: POU class 2 homeobox 1
Protein Name: POU domain, class 2, transcription factor 1 - Aufreinigung
- Immunogen affinity purified.
- Immunogen
- E.coli-derived human Oct-1 recombinant protein (Position: S11-Q240). Human Oct-1 shares 96% and 99% amino acid (aa) sequence identity with mouse and rat Oct-1, respectively.
- Isotyp
- IgG
- Top Product
- Discover our top product POU2F1 Primärantikörper
-
-
- Applikationshinweise
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for Oct-1 is approximately 0.25 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
Notes: Tested Species: Species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. - Kommentare
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
- Beschränkungen
- Nur für Forschungszwecke einsetzbar
-
- Format
- Lyophilized
- Rekonstitution
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- Konzentration
- 500 μg/mL
- Buffer
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
- Konservierungsmittel
- Sodium azide
- Vorsichtsmaßnahmen
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handhabung
- Avoid repeated freezing and thawing.
- Lagerung
- 4 °C/-20 °C
- Informationen zur Lagerung
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.
-
- Target
- POU2F1 (POU Domain, Class 2, Transcription Factor 1 (POU2F1))
- Andere Bezeichnung
- POU2F1 (POU2F1 Produkte)
- Synonyme
- OCT1 antikoerper, OTF1 antikoerper, oct-1B antikoerper, POU2F1 antikoerper, 2810482H01Rik antikoerper, NF-A1 antikoerper, Oct-1 antikoerper, Oct1 antikoerper, Otf-1 antikoerper, Otf1 antikoerper, chOCT-1 antikoerper, oct-1 antikoerper, oct1 antikoerper, pou2f1r antikoerper, fc07d06 antikoerper, wu:fc07d06 antikoerper, zgc:158360 antikoerper, POU class 2 homeobox 1 antikoerper, POU domain, class 2, transcription factor 1 antikoerper, POU class 2 homeobox 1 S homeolog antikoerper, POU class 2 homeobox 1b antikoerper, POU2F1 antikoerper, Pou2f1 antikoerper, pou2f1.S antikoerper, pou2f1b antikoerper
- Hintergrund
-
POU domain, class 2, transcription factor 1, also known as OTF1 or OCT1, is a protein that in humans is encoded by the POU2F1 gene. The POU2F1 transcription factor was among the first identified members of the POU transcription factor family. This gene is mapped to 1q24.1 to q24.2. It has been found that the POU dimer from the POU2F1 gene formed on the palindromic OCT factor recognition element, or PORE (ATTTGAAATGCAAAT), could recruit the transcriptional coactivator OBF1 , whereas POU dimers formed on the consensus MORE (more PORE) (ATGCATATGCAT) or on MOREs from immunoglobulin heavy chain promoters (AT[G/A][C/A]ATATGCAA) failed to interact. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA.
Synonyms: FLJ42836 antibody|NF A1 antibody|NF-A1 antibody|Oct 1 antibody|Oct 1B antibody|Oct-1 antibody|OCT1 antibody|Octamer binding protein 1 antibody|Octamer binding transcription factor 1 antibody|Octamer-binding protein 1 antibody|Octamer-binding transcription factor 1 antibody|OTF 1 antibody|OTF-1 antibody|OTF1 antibody|OTTHUMP00000032348 antibody|OTTHUMP00000032350 antibody|OTTHUMP00000032351 antibody|PO21 antibody|PO2F1 antibody|PO2F1_HUMAN antibody|POU class 2 homeobox 1 antibody|POU domain class 2 transcription factor 1 antibody|POU domain, class 2, transcription factor 1 antibody|POU2F1 antibody - Gen-ID
- 5451
- UniProt
- P14859
-