PAD4 Antikörper (Middle Region)

Produktnummer ABIN3042934

Produktdetails anti-PAD4 Antikörper

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Bindungsspezifität: AA 492-512, Middle Region

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PAD4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: Rabbit IgG polyclonal antibody for Protein-arginine deiminase type-4(PADI4) detection. Tested with WB in Human.

Sequenz: ASPRSCYKLF QEQQNEGHGE A

Kreuzreaktivität (Details): No cross reactivity with other proteins.

Produktmerkmale: Rabbit IgG polyclonal antibody for Protein-arginine deiminase type-4(PADI4) detection. Tested with WB in Human.
Gene Name: peptidyl arginine deiminase, type IV
Protein Name: Protein-arginine deiminase type-4

Aufreinigung: Immunogen affinity purified.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human PADI4(492-512aa ASPRSCYKLFQEQQNEGHGEA).

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, The detection limit for PADI4 is approximately 0.5 ng/lane under reducing conditions.
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested. Optimal dilutions should be determined by end users.

Kommentare:

Antibody can be supported by chemiluminescence kit ABIN921124 in WB.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

Konzentration: 500 μg/mL

Buffer: Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

Konservierungsmittel: Thimerosal (Merthiolate), Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing.

Haltbarkeit: 12 months

Details zu PAD4

Target: PAD4 (PADI4) (Peptidyl Arginine Deiminase, Type IV (PADI4))

Andere Bezeichnung: PADI4 (PADI4 Produkte)

Synonyme: PAD antikoerper, PAD4 antikoerper, PADI5 antikoerper, PDI4 antikoerper, PDI5 antikoerper, Pad4 antikoerper, Pdi4 antikoerper, peptidyl arginine deiminase 4 antikoerper, peptidyl arginine deiminase, type IV antikoerper, PADI4 antikoerper, Padi4 antikoerper

Hintergrund: PADI4(Peptidylarginine Deiminase, Type IV), also known as PAD or PAD4, is a human protein which in humans is encoded by the PADI4 gene. Peptidylarginine deiminases, such as PADI4, make up a family of posttranslational modification enzymes that convert arginine residues to citrulline residues in the presence of calcium ion(Nakashima et al., 1999). Suzuki et al.(2003) found from sequence data that the PADI4 gene maps to 1p36. Cuthbert et al.(2004) described a process, deimination, that converts histone arginine to citrulline and antagonizes arginine methylation. They showed that PADI4 specifically deiminated arg2, arg8, arg17, and arg26 in the H3 tail. Deimination by PADI4 prevented arginine methylation by CARM1. Dimethylation of arginines prevented deimination by PADI4, although monomethylation still allowed deimination to take place. In vivo targeting experiments on an endogenous promoter demonstrated that PADI4 could repress hormone receptor-mediated gene induction. PADI4 was recruited to the pS2 promoter following hormone induction when the gene was transcriptionally downregulated, consistent with a repressive role for PADI4.

Synonyms: EC 3.5.3.15 antibody|HL 60 PAD antibody|HL-60 PAD antibody|PAD 4 antibody|PAD antibody|PADI 4 antibody|PADI 5 antibody|PADI H protein antibody|Padi4 antibody|PADI4_HUMAN antibody|PADI5 antibody|PDI 4 antibody|PDI 5 antibody|PDI4 antibody|PDI5 antibody|Peptidyl arginine deiminase type IV antibody|Peptidyl arginine deiminase type V antibody|Peptidylarginine deiminase IV antibody|Protein arginine deiminase antibody|Protein arginine deiminase type 4 antibody|Protein arginine deiminase type IV antibody|Protein-arginine deiminase type IV antibody|Protein-arginine deiminase type-4 antibody