PKAc Antikörper

Antigen: PKAc

Klonalität: Monoklonal (6D2-1)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN302080

Produktbeschreibung

Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A calpha (PKAc alpha).

Format: Purified

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 6D2-1

Beschreibung: Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitoussignaling pathway important in the cell cycle, cellular communication, memory formation andbehavior. PKA is composed of two catalytic (PKAc, Protein Kinase A catalytic subunit) andtwo regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKAr dimerand two activated PKAc ser/thr protein kinase catalytic monomers. The released PKAc cantranslocate into the nucleus and exert a regulatory role in the activation of multiple nuclearhormone receptors. However, PKAc-mediated activation of tonicity-dependent geneexpression is cAMP independent. Humans express three types of PKAc subunit PKAc alphais present in most human tissues, PKAc beta and gamma are tissue-specific, the later isfound in testes.

Spezifität: The antibody 6D2.1 strongly reacts with human proteinkinase A catalytic (PKAc) alphasubunit, and weakly with PKAc gamma subunit (both around 40 kDa). The recognizedepitope of PKAc alpha is identical between man, sheep, pig, ox and dog.

Anwendungen

Applikationshinweise: Western Blotting - Recommended dilution: 0.5-1 µg/ml. Positive control: HeLa human cervix carcinoma cell line. Application note: To detect PKAc gamma, use a more concentrated lysate from a tissueexpressing this subunit (testis).

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from cell culture supernatant by protein-A affinity chromatography.

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Bilder

Dot Blot analysis of GST and GST-fusion proteins using anti-PKAc (6D2.1) and anti-GST (S-tag-05, cat. no. 11-477-C100).The total amount of material spotted on the nitrocellulose membrane is 5 ng/spot.Lane 1: GST-Akt1Lane 2: GST-Akt2Lane 3: GST-Akt3Lane 4: GST-PKAc alphaLane 5: GST-PKAc betaLane 6: GST-PKAc gammaLane 7: GST-MEK 1Lane 8: GST