TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) Antikörper

Details zu Produkt Nr. ABIN2792680, Anbieter: Anmelden zum Anzeigen
Antigen
  • CG17358
  • Dmel\\CG17358
  • T2DA_DROME
  • TAF
  • TAF12
  • TAF30a
  • TAF30alpha
  • TAF30alpha/28
  • TAF30alpha/28/22
  • TAFII30alpha/22
  • TAF[II]30alpha
  • TAF[[II]]
  • TAF[[II]]230
  • TAF[[II]]30alpha
  • TAF[[II]]30alpha/28 TAF[[II]]22
  • TAF[[II]]30alpha/28/22
  • TFIID
  • TFIID 22
  • TFIID 28
  • TFIID-p28
  • Taf22
  • Taf28
  • Taf30alpha
  • Taf32
  • Taf[[II]]30alpha
  • d28/22
  • dTAF12
  • dTAF22
  • dTAF28
  • dTAFII30
  • dTAFII30 alpha
  • dTAF[[II]]30
  • dTAF[[II]]30alpha
  • dTAF[[II]]30alpha/22
  • dmTAF12
  • p22
  • p28
  • 20kDa
  • 2810422D08Rik
  • AW557038
  • Taf2J
  • TAF2J
  • TAFII20
  • taf12-a
  • TBP-associated factor 12
  • TATA-box binding protein associated factor 12
  • TATA-box binding protein associated factor 12 L homeolog
  • Taf12
  • TAF12
  • taf12.L
Epitop
N-Term
11
3
2
2
1
1
Reaktivität
Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Kaninchen, Ratte (Rattus)
47
28
26
3
3
3
3
2
2
2
2
2
2
1
1
Wirt
Kaninchen
35
12
Klonalität
Polyklonal
Konjugat
Unkonjugiert
3
3
2
1
1
1
1
1
1
1
1
1
1
1
Applikation
Chromatin Immunoprecipitation (ChIP), Western Blotting (WB)
28
20
13
6
5
4
1
1
Optionen
Hersteller
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human TAF12
Sequenz MNQFGPSALI NLSNFSSIKP EPASTPPQGS MANSTAVVKI PGTPGAGGRL
Kreuzreaktivität Rind (Kuh), Hund, Huhn, Human, Maus, Ratte (Rattus)
Homologie Cow: 100%, Dog: 100%, Guinea Pig: 92%, Horse: 100%, Human: 100%, Mouse: 85%, Rabbit: 100%, Rat: 92%
Produktmerkmale This is a rabbit polyclonal antibody against TAF12. It was validated on Western Blot using a cell lysate as a positive control.
Reinigung Affinity Purified
Andere Bezeichnung TAF12 (TAF12 Antibody Abstract)
Hintergrund Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I.Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins. These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site. Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors. Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex. Among the latter are the TAF proteins. Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases. TAF12 interacts directly with TBP as well as with TAF2I. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: TAF2J, TAFII20
Protein Interaction Partner: PRKAR2B, SUMO1, UBE2I, TAF4B, KAT2B, HIST3H3, SUPT3H, TAF4, ERCC1, TAF10, ELAVL1, CPSF1, TAF3, TBP, TAF1A, TAF1C, ATF7, ATXN7, NFYC, NFYB, NFYA, TAF6, TAF8, TAF9, TAF11, TAF5, TAF15, TAF7, TAF2, TAF1, MYC,
Protein Size: 161
Molekulargewicht 18 kDa
Gen-ID 6883
NCBI Accession NM_005644, NP_005635
UniProt Q16514
Forschungsgebiet Chromatin and Nuclear Signaling, Transcription Factors
Applikationshinweise WB Suggested Anti-TAF12 Antibody Titration: 0.2-1 µg/mL
ELISA Titer: 1:312500
Positive Control: Transfected 293T.
Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 161 AA

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) antibody (ABIN2792680) WB Suggested Anti-TAF12 Antibody Titration: 0.2-1 ug/ml
ELISA Titer:
Chromatin Immunoprecipitation (ChIP) image for anti-TAF12 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 20kDa (TAF12) (N-Term) antibody (ABIN2792680) Quiescent human colon carcinoma HCT116 cultures were treated with 10 % FBS for three ...
Allgemeine Veröffentlichungen Denissov, van Driel, Voit, Hekkelman, Hulsen, Hernandez, Grummt, Wehrens, Stunnenberg: "Identification of novel functional TBP-binding sites and general factor repertoires." in: The EMBO journal, Vol. 26, Issue 4, pp. 944-54, 2007 (PubMed).

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