POLR3B Antikörper (C-Term)

Produktnummer ABIN2783516

Produktdetails anti-POLR3B Antikörper

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Bindungsspezifität: C-Term

Reaktivität: Human, Maus, Ratte, Rind (Kuh), Hund, Kaninchen, Zebrafisch (Danio rerio), Pferd, Meerschweinchen, Saccharomyces cerevisiae, Ziege

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser POLR3B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Sequenz: IEKVMISSNA EDAFLIKMLL RQTRRPEIGD KFSSRHGQKG VCGLIVPQED

Homologie: Cow: 100%, Dog: 100%, Goat: 93%, Guinea Pig: 100%, Horse: 100%, Human: 100%, Mouse: 100%, Rabbit: 100%, Rat: 100%, Yeast: 93%, Zebrafish: 100%

Produktmerkmale: This is a rabbit polyclonal antibody against POLR3B. It was validated on Western Blot using a cell lysate as a positive control.

Aufreinigung: Affinity Purified

Immunogen: The immunogen is a synthetic peptide directed towards the C terminal region of human POLR3B

Anwendungsinformationen

Applikationshinweise: Optimal working dilutions should be determined experimentally by the investigator.

Kommentare:

Antigen size: 1133 AA

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freeze-thaw cycles.

Lagerung: -20 °C

Informationen zur Lagerung: For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.

Referenzen für anti-POLR3B Antikörper (ABIN2783516)

Yee, Gong, Huang, Lorent, Dolan, Maraia, Pack: "Mutation of RNA Pol III subunit rpc2/polr3b Leads to Deficiency of Subunit Rpc11 and disrupts zebrafish digestive development." in: PLoS biology, Vol. 5, Issue 11, pp. e312, (2007) (PubMed).

Details zu POLR3B

Target: POLR3B (Polymerase (RNA) III (DNA Directed) Polypeptide B (POLR3B))

Andere Bezeichnung: POLR3B (POLR3B Produkte)

Synonyme: RGD1565311 antikoerper, C128 antikoerper, HLD8 antikoerper, RPC2 antikoerper, 2700078H01Rik antikoerper, A330032P03Rik antikoerper, C85372 antikoerper, RNA polymerase III subunit B antikoerper, polymerase (RNA) III (DNA directed) polypeptide B antikoerper, Polr3b antikoerper, POLR3B antikoerper

Hintergrund: POLR3B belongs to the RNA polymerase beta chain family. DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3B is the second largest core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It is proposed to contribute to the polymerase catalytic activity and forms the polymerase active center together with the largest subunit. Pol III is composed of mobile elements and RPC2 is part of the core element with the central large cleft and probably a clamp element that moves to open and close the cleft.
Alias Symbols: FLJ10388, RPC2, C128, HLD8
Protein Interaction Partner: SUMO2, UBC, POLR3H, TSR1, POLR1D, CRCP, POLR3A, POLR1C, POLR2H, POLR3D, RPAP2, RPAP1, GPN1, RAE1,
Protein Size: 1133

Molekulargewicht: 128 kDa

Gen-ID: 55703

NCBI Accession: NM_018082, NP_060552

UniProt: Q9NW08