Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (N-Term) Antikörper

Details zu Produkt Nr. ABIN2782428, Anbieter: Anmelden zum Anzeigen
Antigen
  • p40phox
  • AI451400
  • NCF
  • P40PHOX
  • SH3PXD4
  • p40phox protein
  • neutrophil cytosolic factor 4
  • neutrophil cytosolic factor 4, 40kDa
  • p40phox
  • LOC100136241
  • Ncf4
  • NCF4
Epitop
N-Term
19
9
9
4
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reaktivität
Rind (Kuh), Hund, Meerschweinchen, Pferd, Human, Maus, Schwein, Kaninchen, Ratte (Rattus)
117
57
43
6
6
4
3
3
3
2
Wirt
Kaninchen
90
20
11
Klonalität
Polyklonal
Konjugat
Unkonjugiert
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
Applikation
Western Blotting (WB)
74
41
33
26
23
5
4
3
3
2
2
1
Optionen
Hersteller
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Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human NCF4
Blocking Peptide Zu diesem Produkt passt das Blocking Peptide ABIN980522.
Sequenz SKYLIYRRYR QFHALQSKLE ERFGPDSKSS ALACTLPTLP AKVYVGVKQE
Homologie Cow: 85%, Dog: 86%, Guinea Pig: 79%, Horse: 86%, Human: 100%, Mouse: 92%, Pig: 92%, Rabbit: 85%, Rat: 92%
Produktmerkmale This is a rabbit polyclonal antibody against NCF4. It was validated on Western Blot using a cell lysate as a positive control.
Reinigung Affinity Purified
Andere Bezeichnung NCF4 (NCF4 Antibody Abstract)
Hintergrund NCF4 is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense. This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation. This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of the PI(3) kinase, which suggests its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein was found to negatively regulate the enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Alias Symbols: MGC3810, NCF, P40PHOX, SH3PXD4
Protein Interaction Partner: NCF2, UBC, CORO1A, MSN, CYBB, NCF1, CYBA, TXN, PRKCD, XRCC6, PRKDC,
Protein Size: 348
Molekulargewicht 39 kDa
Gen-ID 4689
NCBI Accession NM_013416, NP_038202
UniProt A8K4F9
Forschungsgebiet Metabolism
Applikationshinweise Optimal working dilutions should be determined experimentally by the investigator.
Kommentare

Antigen size: 348 AA

Beschränkungen Nur für Forschungszwecke einsetzbar
Format Liquid
Konzentration Lot specific
Buffer Liquid. Purified antibody supplied in 1x PBS buffer with 0.09 % (w/v) sodium azide and 2 % sucrose.
Konservierungsmittel Sodium azide
Vorsichtsmaßnahmen This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung Avoid repeated freeze-thaw cycles.
Lagerung -20 °C
Informationen zur Lagerung For short term use, store at 2-8°C up to 1 week. For long term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Bilder des Herstellers
Western Blotting (WB) image for anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (N-Term) Antikörper (ABIN2782428) anti-Neutrophil Cytosolic Factor 4, 40kDa (NCF4) (N-Term) antibody
Allgemeine Veröffentlichungen Chen, He, Minshall, Dinauer, Ye: "Characterization of a mutation in the Phox homology domain of the NADPH oxidase component p40phox identifies a mechanism for negative regulation of superoxide production." in: The Journal of biological chemistry, Vol. 282, Issue 41, pp. 30273-84, 2007 (PubMed).