Cardiac Troponin T2 Antikörper

Produktnummer ABIN2688865

Produktdetails anti-Cardiac Troponin T2 Antikörper

Target: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Reaktivität: Human, Maus, Ratte, Schwein, Kaninchen, Hund, Huhn, Meerschweinchen

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser Cardiac Troponin T2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Intracellular Staining (ICS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Marke: BD Pharmingen™

Produktmerkmale: The 13-11 monoclonal antibody specifically recognizes Troponin T type 2 (cardiac), encoded by the gene TNNT2. Troponin T is the tropomyosin-binding subunit of the troponin complex, which also encompasses troponin C and troponin I. This complex regulates muscle contraction in skeletal and cardiac muscle in response to alterations in calcium levels. Troponin T type 2 is solely found in the heart, and genetic alterations in the TNNT2 gene are associated to a series of heart disorders in humans, including hypertrophic cardiomyopathy, dilated cardiomyopathy and left ventricular noncompaction. Cardiac Troponin T can be used as a marker for the identification of cardiomyocytes derived from pluripotent stem cells. LEFT PANEL: Western blot analysis of Cardiac Troponin T expression in human and mouse tissues. Human heart lysate was obtained from Abcam (Cat .No. ab2943, lane 1). C2C12 mouse myoblasts (ATCC CRL-1772) were cultured in DMEM containing 10 % FBS (lane 2) or 0.5 % FBS for 14 days for induction of cell differentiation (lane 3). Lysates were prepared, electrophoresed (SDS-PAGE) and transferred to PVDF membranes. The membranes were labeled with 1 μg/mL of Purified Mouse Anti-Cardiac Troponin T. Specific staining was detected with HRP Goat Anti-Mouse Ig (Cat. No. 554002). RIGHT PANEL: Immunohistochemical staining of Cardiac Troponin T in human heart. Following antigen retrieval with BD Retrievagen A Buffer (Cat. No. 550524), the formalin-fixed paraffin-embedded sections were stained with either Purified Mouse IgG1 κ Isotype Control (Cat. No.550878, left) or Purified Mouse Anti-Cardiac Troponin T (right). A three-step staining procedure that employs Biotin Goat Anti-Mouse Immunoglobulin (Cat. No. 550337), Streptavidin HRP (Cat. No.550946), and the DAB Substrate Kit (Cat. No. 550880) was used to develop the primary staining reagents. Original magnification: 40x. Flow cytometric analysis of Cardiac Troponin T in human (left panel) and mouse (right panel) cardiomyocytes differentiated in vitro. Human embryonic stem cell-derived cardiomyocytes (Evans lab, UCSD) were disassociated and fixed in BD Cytofix™ Fixation Buffer (Cat. No. 554655) and permeabilized with BD Phosflow™ Perm/Wash buffer I (Cat. No. 557885). The C2C12 mouse myoblast cell line (ATCC CRL-1772) was cultured for 5 days in low-serum conditions for induction of cell differentiation, fixed with BD Cytofix™ Fixation Buffer, and permeabilized with BD Perm/Wash™ Buffer (Cat. No. 554723). The cells were stained with either Purified Mouse IgG1, κ Isotype Control (Cat. No. 554121, dashed line) or Purified Mouse Anti-Cardiac Troponin T (solid line). The second-step reagents were FITC Goat Anti-Mouse Ig (Cat. No. 554001, left) and PE Goat Anti-Mouse Ig (Cat. No. 550589, right). Events with the forward and side light-scatter characteristics of intact cells were gated. Flow cytometry was performed on a BD LSR Fortessa™ (left) or a BD FACSCanto™ II (right) flow cytometry system.

BD Pharmingen™ Purified Mouse Anti-Cardiac Troponin T - Purified - Clone 13-11 - Isotype Mouse IgG1, κ - Reactivity Ms, Hu, Chick, Dog, G Pig, Pig, Rab, Rat - 0.1 mg

Aufreinigung: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rabbit cardiac troponin T Protein

Klon: 13

Isotyp: IgG1 kappa

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Konzentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C

Informationen zur Lagerung: Store undiluted at 4°C.

Referenzen für anti-Cardiac Troponin T2 Antikörper (ABIN2688865)

Jáchymová, Muravská, Paleček, Kuchynka, Řeháková, Magage, Kr��l, Zima, Horký, Linhart: "Genetic variation screening of TNNT2 gene in a cohort of patients with hypertrophic and dilated cardiomyopathy." in: Physiological research / Academia Scientiarum Bohemoslovaca, Vol. 61, Issue 2, pp. 169-75, (2012) (PubMed).

Lian, Hsiao, Wilson, Zhu, Hazeltine, Azarin, Raval, Zhang, Kamp, Palecek: "Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 109, Issue 27, pp. E1848-57, (2012) (PubMed).

Uosaki, Fukushima, Takeuchi, Matsuoka, Nakatsuji, Yamanaka, Yamashita: "Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression." in: PLoS ONE, Vol. 6, Issue 8, pp. e23657, (2011) (PubMed).

Zhang, Wilson, Soerens, Koonce, Yu, Palecek, Thomson, Kamp: "Functional cardiomyocytes derived from human induced pluripotent stem cells." in: Circulation research, Vol. 104, Issue 4, pp. e30-41, (2009) (PubMed).

Mauritz, Schwanke, Reppel, Neef, Katsirntaki, Maier, Nguemo, Menke, Haustein, Hescheler, Hasenfuss, Martin: "Generation of functional murine cardiac myocytes from induced pluripotent stem cells." in: Circulation, Vol. 118, Issue 5, pp. 507-17, (2008) (PubMed).

Thierfelder, Watkins, MacRae, Lamas, McKenna, Vosberg, Seidman, Seidman: "Alpha-tropomyosin and cardiac troponin T mutations cause familial hypertrophic cardiomyopathy: a disease of the sarcomere." in: Cell, Vol. 77, Issue 5, pp. 701-12, (1994) (PubMed).

Malouf, McMahon, Oakeley, Anderson: "A cardiac troponin T epitope conserved across phyla." in: The Journal of biological chemistry, Vol. 267, Issue 13, pp. 9269-74, (1992) (PubMed).

Details zu Cardiac Troponin T2

Target: Cardiac Troponin T2 (cTnT) (Cardiac Troponin T (cTnT))

Andere Bezeichnung: Cardiac Troponin T (cTnT Produkte)

Synonyme: CMH2 antikoerper, CMPD2 antikoerper, LVNC6 antikoerper, RCM3 antikoerper, TnTC antikoerper, cTnT antikoerper, Tnt antikoerper, CTTG antikoerper, Ctt antikoerper, RATCTTG antikoerper, Tnnt3 antikoerper, tnnt2 antikoerper, CT3 antikoerper, si:ch211-136g2.1 antikoerper, troponin T2, cardiac type antikoerper, troponin T2, cardiac antikoerper, troponin T type 2a (cardiac) antikoerper, troponin T2, cardiac type L homeolog antikoerper, troponin T2e, cardiac antikoerper, TNNT2 antikoerper, Tnnt2 antikoerper, tnnt2a antikoerper, tnnt2.L antikoerper, tnnt2e antikoerper

Hintergrund: Synonyms: cTnT, Cardiac Muscle Troponin T, Troponin T type 2, TNNT2

Molekulargewicht: 35-40 kDa