Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Aufreinigung
VPS25 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
VPS25 antibody was raised against a 10 amino acid synthetic peptide near the internal region of VPS25.
VPS25
Reaktivität: Human
ELISA
Wirt: Kaninchen
Polyclonal
Biotin
Applikationshinweise
ELISA: Antibody detection limit dilution 1:1,000. Western Blot: Approximately 20-22 kDa kDa band observed in human duodenum, kidney and liver lysates (calculated MW of 20.7 kDa according to NP_115729.1). Recommended concentration: 0.5-1.5 μg/mL.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
VPS25
(Vacuolar Protein-Sorting-Associated Protein 25 (VPS25))