GRAF antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
GRAF antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of GRAF.
ARHGAP26
Reaktivität: Human, Maus, Ratte
WB, IF (cc), IF (p)
Wirt: Kaninchen
Polyclonal
Alexa Fluor 350
Applikationshinweise
ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 90 kDa band observed in human heart lysates (calculated MW of 92.2 kDa according to NP_055886.1). Recommended concentration: 0.3-1 μg/mL. an additional strong band of unknown identity was also consistently observed at 16 kDa . This band was successfully blocked by incubation with the immunizing peptide.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Format
Liquid
Konzentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Konservierungsmittel
Sodium azide
Vorsichtsmaßnahmen
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handhabung
Minimize freezing and thawing.
Lagerung
-20 °C
Informationen zur Lagerung
Aliquot and store at -20 °C.
Target
GRAF (ARHGAP26)
(rho GTPase Activating Protein 26 (ARHGAP26))