Protein Kinase A, alpha (PRKACA) Antikörper

Antigen: Protein Kinase A, alpha (PRKACA)

Klonalität: Monoklonal (6D2-1)

Wirt: Maus

Reaktivität: Human

Applikation: Western Blot (WB)

Produktnummer: ABIN238374

Produktbeschreibung

Produktmerkmale: Synonyms: PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha

Weitere Bezeichnung: PRKACA

Swiss-Prot: P17612

Immunogen: Peptide corresponding to amino acids ESPAQNTAHLDQFERIK of human proteinkinase A calpha (PKAc alpha).

Kreuzreaktivität: Human

Isotyp: IgG1  (Passende Sekundärantikörper)

Klon: 6D2-1

Beschreibung: Protein kinase A (PKA, cAMP-dependent protein kinase) is a key element of a ubiquitoussignaling pathway important in the cell cycle, cellular communication, memory formationand behavior. PKA is composed of two catalytic (PKAc, Protein Kinase A catalytic subunit)and two regulatory subunits (PKAr). Upon binding cAMP, the complex dissociates to PKArdimer and two activated PKAc ser/thr protein kinase catalytic monomers. The releasedPKAc can translocate into the nucleus and exert a regulatory role in the activation ofmultiple nuclear hormone receptors. However, PKAc-mediated activation oftonicity-dependent gene expression is cAMP independent. Humans express three types ofPKAc subunit - PKAc alpha is present in most human tissues, PKAc beta and gamma aretissue-specific, the later is found in testes.

Spezifität: This antibody strongly reacts with human proteinkinase A catalytic (PKAc) alpha subunit,and weakly with PKAc gamma subunit (both around 40 kDa). The recognized epitope of PKAc alpha is identical between man, sheep, pig, ox and dog. Species: Human. Others not tested.

Anwendungen

Applikationshinweise: Suitable for Western Blotting (0.5-1 μg/ml). Positive control: HeLa human cervix carcinoma cell lineNote: To detect PKAc gamma, use a more concentrated lysate from a tissue expressing thissubunit (testis). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.

Konzentration: 1.0 mg/ml

Reinigung: Aff - Purified

Buffer: PBS, pH 7.4 with 15 mM sodium azide as preservative.

Lagerung: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid freeze/thaw cycles. Shelf life: One year from despatch.

Forschungsgebiet: Krebs, Kinasen/Phosphatasen

Beschränkungen: Nur für Forschungszwecke einsetzbar