GLUT4 Antikörper

Produktnummer ABIN218433

Der Kaninchen Polyklonal anti-GLUT4 Antikörper (ABIN218433) detektiert spezifisch GLUT4 in WB, IHC, IP, IHC (p) und IHC (fro). Dieser Antikörper reagiert spezifisch mit Proben aus Human, Maus, Ratte und Affe.

Kurzübersicht für GLUT4 Antikörper (ABIN218433)

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Reaktivität: Human, Maus, Ratte, Affe

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLUT4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Produktdetails anti-GLUT4 Antikörper

Spezifität: Recognizes human Solute carrier family 2, facilitated glucose transporter member 4, also known as glucose transporter 4 or GLUT4. GLUT4 is a 509 amino acid ~50 kD multi-pass transmembrane protein located primarily in the perinuclear region and is translocated to the plasma membrane where it is rapidly reinternalized. Does not react with HEP G2 type glucose transporter in human erythrocytes, rat brain or other tissues that do not exhibit sensitivity to insulin. GLUT4 is the insulin-regulated glucose transporter found in adipose tissue and striated muscle, responsible for glucose disposal. The stimulation of glucose uptake by insulin requires translocation of the GLUT4 glucose transporter from intracellular storage sites to the cell surface. Activation of phosphatidylinositol-3-OH kinase (PI3K) is required for this trafficking event, but it is not sufficient to produce GLUT4 translocation. Human insulin resistance involves a defect in GLUT4 traffic and targeting leading to accumulation in a dense membrane compartment from which insulin is unable to recruit GLUT4 to the cell surface.

Keine Kreuzreaktivität: Schwein

Aufreinigung: Affinity purified

Immunogen: A 12 amino acid peptide corresponding to the carboxyl terminus of the insulin regulatable glucose transporter  conjugated to keyhole limpet hemocyanin.

Type of Immunogen: Synthetic peptide - KLH conjugated

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr, IHC-P (10 - 20 μg/mL), IP, WB

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10-20 μg/mL.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles.

Details zu GLUT4

Target: GLUT4 (SLC2A4) (Solute Carrier Family 2 (Facilitated Glucose Transporter), Member 4 (SLC2A4))

Andere Bezeichnung: SLC2A4 / GLUT4 / GLUT-4

Hintergrund: Name/Gene ID: SLC2A4
Subfamily: Sugar transporter
Family: Transporter

Synonyms: SLC2A4, Glucose transporter 4, Glucose transporter GLUT4, GLUT4, GLUT-4

Gen-ID: 6517

UniProt: P14672

Pathways: AMPK Signaling, Carbohydrate Homeostasis, Proton Transport, Brown Fat Cell Differentiation, Warburg Effekt