MDM2 Antikörper (AA 154-167)

Produktnummer ABIN214211

Der Maus Monoklonal anti-MDM2 Antikörper wird verwendet zum Nachweis von MDM2 in Proben von Human, Ratte, Affe und Hund. Er wurde validiert für IHC, IHC (p), IP, IHC (fro) und IHC (rs).

Kurzübersicht für MDM2 Antikörper (AA 154-167) (ABIN214211)

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Reaktivität: Human, Ratte, Affe, Hund

Wirt: Maus

Klonalität: Monoklonal

Konjugat: Dieser MDM2 Antikörper ist unkonjugiert

Applikation: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Resin Sections) (IHC (rs))

Klon: SMP14

Produktdetails anti-MDM2 Antikörper

Bindungsspezifität: AA 154-167

Spezifität: Recognizes the 90kD MDM2 protein, which forms a complex with p53 leading to p53 inhibition. MDM2 also acts to stimulate cell proliferation via its induction of transcription factors such as E2F1 and DPI. MDM2 is over expressed in a range of human malignancies, including soft tissue sarcomas, breast cancer and bladder cancer.

Homologie: Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Dog, Panda (100%) Bat, Cat, Bovine, Hamster, Elephant, Horse, Pig (93%) Mouse (86%).

Aufreinigung: Purified IgG

Immunogen: Synthetic peptide, CSRPSTSSRRRAISE, corresponding to amino acids 154-167 of human MDM2. Percent identity by BLAST analysis: Human, Orangutan, Gibbon, Monkey, Marmoset, Rat, Dog, Panda (100%), Bat, Cat, Bovine, Hamster, Elephant, Horse, Pig (93%), Mouse (86%).

Type of Immunogen: Synthetic peptide

Isotyp: IgG1

Anwendungsinformationen

Applikationshinweise: Approved: IHC, IHC-Fr, IHC-P (10 μg/mL), IHC-R, IP

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Histology Positive Control Tissue: Breast Carcinoma.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: PBS, 0.09 % sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeated freezing and thawing. Store undiluted.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C or -20°C, Avoid freeze-thaw cycles. Store undiluted.

Details zu MDM2

Target: MDM2 (Mdm2, p53 E3 Ubiquitin Protein Ligase Homolog (Mouse) (MDM2))

Andere Bezeichnung: MDM2

Hintergrund: Name/Gene ID: MDM2

Synonyms: MDM2, ACTFS, Double minute 2 protein, Hdm2, MDM2 variant FB30, HDMX, Oncoprotein Mdm2, p53-binding protein Mdm2, MDM2 variant FB28

Gen-ID: 4193

Pathways: p53 Signalweg, PI3K-Akt Signalweg, Zellzyklus, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Autophagie, Ubiquitin Proteasome Pathway