STAT1 Antikörper (C-Term)

Produktnummer ABIN212695

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch STAT1 in WB und IP. Es zeigt Reaktivität gegenüber Proben von Human, Maus und Ratte.

Kurzübersicht für STAT1 Antikörper (C-Term) (ABIN212695)

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser STAT1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunoprecipitation (IP)

Produktdetails anti-STAT1 Antikörper

Bindungsspezifität: C-Term

Spezifität: Recognizes the STAT1 protein, Mr 91kD. of human, mouse and rat. Other species cross-reactivity is unknown.

Aufreinigung: Protein A purified

Immunogen: GST fusion-protein containing the carboxy terminal 36 amino acids of human STAT1a. This sequence is missing in the b isoform.

Type of Immunogen: GST fusion protein

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Approved: IP, WB (0.5 - 2 μg/mL)

Usage: Suitable for use in Western Blot, Immunoprecipitation. Western Blot: 0.5-2 μg/mL detects STAT1 in RIPA lysates from human Jurkat, mouse CTLL and rat L6 cells. Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-STAT1 (1 μg/mL). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Immunoprecipitation: 4 mg immunoprecipitated STAT1 from 500 mg of human Jurkat RIPA lysate.

Kommentare:

Target Species of Antibody: Human

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: Lot specific

Buffer: 250 μLof 0.07 M Tris-glycine, 0.105 M sodium chloride, 0.035 % sodium azide, 30 % glycerol.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Avoid repeat freeze-thaw cycles.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Details zu STAT1

Target: STAT1 (Signal Transducer and Activator of Transcription 1, 91kDa (STAT1))

Andere Bezeichnung: STAT1

Hintergrund: Name/Gene ID: STAT1

Synonyms: STAT1, ISGF-3, STAT91, CANDF7

Gen-ID: 6772

UniProt: P42224

Pathways: JAK-STAT Signalweg, RTK Signalweg, Interferon-gamma Pathway, Response to Growth Hormone Stimulus, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, CXCR4-mediated Signaling Events