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Antikörper
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anti-Integrin alpha M ITGAM
Integrin alpha M (ITGAM) Antikörper
| Antigen |
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| Synonyme |
CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik |
| Klonalität |
Monoklonal (MEM-170) |
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Wirt
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Reaktivität
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Human (238), Maus (205), Ratte (Rattus) (41), Kaninchen (25), Affe (21), Schaf (20), Schwein (19), Pavian (17), Cynomolgus (17), Rhesusaffen (17), Rind (Kuh) (13), Hund (6), Ziege (4), Primaten (3), Pferd (2), Katze (1), Marder (1)
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Applikation
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 Alternativen Durchflusszytometrie (FACS), Funktionale Studien (Func)
Durchflusszytometrie (FACS) (328), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (101), Immunpräzipitation (IP) (87), Western Blot (WB) (30), Immunfluoreszenz (IF) (21), Immunhistochemie (IHC) (20), Funktionale Studien (Func) (19), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Blockierende Antikörper (Inhibition) (17), Enzyme Immunoassay (EIA) (7), ELISA (3), Immunzytochemie (ICC) (3), Radioimmunoassay (RIA) (3), Dot Blot (Dot) (2)
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| Produktnummer |
ABIN192410 |
| Menge |
0.1 mg (1 mg/ml) |
| Preis |
180,00 € Zzgl. Versandkosten €20,00 und MWSt
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| Lieferung nach |
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| Verfügbarkeit |
Lieferbar innerhalb von 3 bis 5 Werktagen |
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Weitere Bezeichnung
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CD11b
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Immunogen
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Human granulocytes
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Format
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Azide Free
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Isotyp
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IgG1 (Passende Sekundärantikörper)
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Klon
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MEM-170
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Beschreibung
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CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein thatnon-covalently associates with integrin beta2 subunit (CD18), expression of the CD11bchain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin(Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less onmacrophages. CD11b/CD18 integrin is implicated in various adhesive interactions ofmonocytes, macrophages and granulocytes, facilitating their diapedesis, as well as itmediates the uptake of complement coated particles, serving as a receptor for the iC3bfragment of the third complement component.
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Spezifität
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The antibody MEM-170 recognizes CD11b antigen (Mac-1alpha), a 165-170 kDatransmembrane protein type 1 mainly expressed on monocytes, granulocytes and NK-cells.The antibody blocks (not completely) Mac-1-mediated neutrophil binding to fibrinogen.HLDA VI., WS Code A054
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Applikationshinweise
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Functional Application: The antibody MEM-170 blocks (not completely) Mac-1-mediated neutrophil binding tofibrinogen.
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Konzentration
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1 mg/ml
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Reinheit
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> 95% (by SDS-PAGE)
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Reinigung
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Purified from ascites by protein-G affinity chromatography.
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Buffer
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Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.
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Lagerung
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Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.
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Forschungsgebiet
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Stammzellen, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, Glia Marker, CD Antigene, Oberflächenrezeptoren der Immunzellen
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Beschränkungen
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Nur für Forschungszwecke einsetzbar
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Alternativen zu Antigen "Integrin alpha M (ITGAM)", Typ "Antikörper" finden
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Wirte
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Ratte (184), Maus (170), Kaninchen (31), Ziege (7)
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Reaktivitäten
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Human (238), Maus (205), Ratte (Rattus) (41), Kaninchen (25), Affe (21), Schaf (20), Schwein (19), Pavian (17), Cynomolgus (17), Rhesusaffen (17), Rind (Kuh) (13), Hund (6), Ziege (4), Primaten (3), Pferd (2), Katze (1), Marder (1)
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Applikationen
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Durchflusszytometrie (FACS) (328), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (101), Immunpräzipitation (IP) (87), Western Blot (WB) (30), Immunfluoreszenz (IF) (21), Immunhistochemie (IHC) (20), Funktionale Studien (Func) (19), Immunhistochemie (Paraffinschnitte) (IHC (p)) (18), Blockierende Antikörper (Inhibition) (17), Enzyme Immunoassay (EIA) (7), ELISA (3), Immunzytochemie (ICC) (3), Radioimmunoassay (RIA) (3), Dot Blot (Dot) (2)
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Konjugate
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FITC (56), PE (44), Biotin (41), APC (14), PE/Cy5 (8), PE/Cy7 (8), Cy5 (7), Alexa Fluor 488 (6), Alexa Fluor 647 (6), APC/Cy7 (4), Alexa Fluor 700 (4), Pacific Blue (4), PerCP/Cy5.5 (4), RPE (4), SPRD (4), PerCP (2), APC,Cy7 (1), HRP (1), PE,Cy5 (1), PE,DY647 (1), PE-Dyomics 647 (1), RPE,Cy5 (1)
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Epitope
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C-Term (1)
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