TCF3 Antikörper (Internal Region)

Produktnummer ABIN190880

Der Ziege Polyklonal anti-TCF3 Antikörper wird verwendet zum Nachweis von TCF3 in Proben von Human. Er wurde validiert für WB, ELISA und IHC.

Kurzübersicht für TCF3 Antikörper (Internal Region) (ABIN190880)

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser TCF3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Güteklasse: Verified

Produktdetails anti-TCF3 Antikörper

Bindungsspezifität: Internal Region

Verwendungszweck: TCF3 / ITF1

Sequenz: KAPRARTSPD EDED

Spezifität: This antibody is expected to recognize reported isoform E12 (NP_003191.1) only.

Kreuzreaktivität: Human

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-KAPRARTSPDEDED, from the internal region of the protein sequence according to NP_003191.1.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Immunohistochemistry: In paraffin embedded Human Kidney shows strong nuclear staining in DCT cells. Recommended concentration, 3-5 μg/mL.

Western Blot: Approx 75 kDa band observed in lysates of cell line Daudi (calculated MW of 67.6 kDa according to NP_003191.1). Recommended concentration: 0.01-0.03 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:128000.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu TCF3

Target: TCF3 (Transcription Factor 3 (E2A Immunoglobulin Enhancer Binding Factors E12/E47) (TCF3))

Andere Bezeichnung: TCF3

Hintergrund: TCF3, ITF1, transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47), E2A, MGC129647, MGC129648 , E2A immunoglobulin enhancer-binding factor E12/E47, immunoglobulin transcription factor 1, kappa-E2-binding factor, transcription factor

Gen-ID: 6929

NCBI Accession: NP_003191

Pathways: WNT Signalweg, Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Production of Molecular Mediator of Immune Response