PCK1 Antikörper (C-Term)

Produktnummer ABIN1882180

Produktdetails anti-PCK1 Antikörper

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Bindungsspezifität: AA 592-622, C-Term

Reaktivität: Human, Maus, Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PCK1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Aufreinigung: This antibody is purified through a protein A column, followed by peptide affinity purification.

Immunogen: This PCK1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 592-622 amino acids from the C-terminal region of human PCK1.

Klon: RB03646

Isotyp: Ig Fraction

Anwendungsinformationen

Applikationshinweise: IF: 1:10~50. WB: 1:1000. WB: 1:1000. WB: 1:2000. IHC-P-Leica: 1:250

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Buffer: Purified polyclonal antibody supplied in PBS with 0.09 % (W/V) sodium azide.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: 4 °C,-20 °C

Haltbarkeit: 6 months

Referenzen für anti-PCK1 Antikörper (ABIN1882180)

Gummadi, Taylor, Curthoys: "Concurrent binding and modifications of AUF1 and HuR mediate the pH-responsive stabilization of phosphoenolpyruvate carboxykinase mRNA in kidney cells." in: American journal of physiology. Renal physiology, Vol. 303, Issue 11, pp. F1545-54, (2012) (PubMed).

Berger, Lindh, Wierup, Zmuda-Trzebiatowska, Lindqvist, Manganiello, Degerman: "Phosphodiesterase 3B is localized in caveolae and smooth ER in mouse hepatocytes and is important in the regulation of glucose and lipid metabolism." in: PLoS ONE, Vol. 4, Issue 3, pp. e4671, (2009) (PubMed).

Franckhauser, Muñoz, Elias, Ferre, Bosch: "Adipose overexpression of phosphoenolpyruvate carboxykinase leads to high susceptibility to diet-induced insulin resistance and obesity." in: Diabetes, Vol. 55, Issue 2, pp. 273-80, (2006) (PubMed).

Dunten, Belunis, Crowther, Hollfelder, Kammlott, Levin, Michel, Ramsey, Swain, Weber, Wertheimer: "Crystal structure of human cytosolic phosphoenolpyruvate carboxykinase reveals a new GTP-binding site." in: Journal of molecular biology, Vol. 316, Issue 2, pp. 257-64, (2002) (PubMed).

Strausberg, Feingold, Grouse, Derge, Klausner, Collins, Wagner, Shenmen, Schuler, Altschul, Zeeberg, Buetow, Schaefer, Bhat, Hopkins, Jordan, Moore, Max, Wang, Hsieh, Diatchenko, Marusina, Farmer et al.: "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. ..." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 99, Issue 26, pp. 16899-903, (2002) (PubMed).

Deloukas, Matthews, Ashurst, Burton, Gilbert, Jones, Stavrides, Almeida, Babbage, Bagguley, Bailey, Barlow, Bates, Beard, Beare, Beasley, Bird, Blakey, Bridgeman, Brown, Buck, Burrill, Butler, Carder et al.: "The DNA sequence and comparative analysis of human chromosome 20. ..." in: Nature, Vol. 414, Issue 6866, pp. 865-71, (2002) (PubMed).

OBrien, Printz, Halmi, Tiesinga, Granner: "Structural and functional analysis of the human phosphoenolpyruvate carboxykinase gene promoter." in: Biochimica et biophysica acta, Vol. 1264, Issue 3, pp. 284-8, (1996) (PubMed).

Ting, Burgess, Chamberlain, Keith, Falls, Meisler: "Phosphoenolpyruvate carboxykinase (GTP): characterization of the human PCK1 gene and localization distal to MODY on chromosome 20." in: Genomics, Vol. 16, Issue 3, pp. 698-706, (1993) (PubMed).

Details zu PCK1

Target: PCK1 (phosphoenolpyruvate Carboxykinase 1 (Soluble) (PCK1))

Andere Bezeichnung: PCK1 (PCK1 Produkte)

Synonyme: PEPCK-C antikoerper, PEPCK1 antikoerper, PEPCKC antikoerper, PEPCK antikoerper, PEPCK-M antikoerper, PEPCK2 antikoerper, 1810010O14Rik antikoerper, 9130022B02Rik antikoerper, cb856 antikoerper, cb924 antikoerper, fj93h11 antikoerper, zgc:63869 antikoerper, wu:fc51c05 antikoerper, wu:fj93h11 antikoerper, pepck-c antikoerper, pepck1 antikoerper, pepckc antikoerper, PCK1 antikoerper, PPCK1 antikoerper, AI265463 antikoerper, Pck-1 antikoerper, GTP antikoerper, PCK antikoerper, Pepck antikoerper, RATPEPCK antikoerper, Ppc1C antikoerper, PHOSPHOENOLPYRUVATE CARBOXYKINASE antikoerper, T28I19.150 antikoerper, T28I19_150 antikoerper, phosphoenolpyruvate carboxykinase 1 antikoerper, 143299_at antikoerper, CG10924 antikoerper, CG17725 antikoerper, Dmel\\CG17725 antikoerper, Dromel_CG17725_FBtr0086701_pepck_mORF antikoerper, dPEPCK antikoerper, pepck antikoerper, PEPC antikoerper, PEPCase antikoerper, ppc antikoerper, phosphoenolpyruvate carboxykinase 1 antikoerper, phosphoenolpyruvate carboxykinase 2, mitochondrial antikoerper, phosphoenolpyruvate carboxykinase 2 (mitochondrial) antikoerper, phosphoenolpyruvate carboxykinase 1 (soluble) antikoerper, phosphoenolpyruvate carboxykinase 1 S homeolog antikoerper, phosphoenolpyruvate carboxykinase, cytosolic [GTP] antikoerper, phosphoenolpyruvate carboxykinase 1, cytosolic antikoerper, phosphoenolpyruvate carboxylase 7 antikoerper, Phosphoenolpyruvate carboxykinase antikoerper, phosphoenolpyruvate carboxylase antikoerper, PCK1 antikoerper, PCK2 antikoerper, Pck2 antikoerper, pck1 antikoerper, pck1.S antikoerper, LOC100634531 antikoerper, Pck1 antikoerper, pep7 antikoerper, Pepck antikoerper, LOC107777405 antikoerper

Hintergrund: This gene is a main control point for the regulation of gluconeogenesis. The cytosolic enzyme encoded by this gene, along with GTP, catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. The expression of this gene can be regulated by insulin, glucocorticoids, glucagon, cAMP, and diet. A mitochondrial isozyme of the encoded protein also has been characterized.

Molekulargewicht: 69195

NCBI Accession: NP_002582

UniProt: P35558

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis