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Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) (internal) Antikörper
| Antigen | Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2) |
| Synonyme | CD, ACUG, BLAU, IBD1, NLRC2, NOD2B, CARD15, CLR16.3, PSORAS1 |
| Epitope |
Alternativen internal |
| Klonalität | Polyklonal |
| Wirt |
Alternativen Ziege |
| Reaktivität |
Alternativen Human |
| Applikation |
Alternativen ELISA, Western Blot (WB)
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1 Publikation vorhanden |
| Produktnummer | ABIN185703 |
| Menge | 100 ug |
| Preis | 286,00 € Zzgl. Versandkosten €40,00 und MWSt |
| Lieferung nach |
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| Verfügbarkeit | Lieferung in 5 Werktagen |
Produktbeschreibung
| Weitere Bezeichnung | CARD15 / NOD2 (Internal) |
| Gen-ID | 64127, 257632 (mouse), 291912 (rat) |
| Immunogen | KFRFTDRERHCSPTD |
| Format | Affinity-purified |
| Beschreibung | All names and symbols: CARD15, NOD2, caspase recruitment domain family, member 15, ACUG, BLAU, CD, IBD1, NOD2B, PSORAS1, LRR-containing protein, NOD2 protein, caspase recruitment domain protein 15, inflammatory bowel disease protein 1, nucleotide-binding oligomerization domain 2,. Official Symbol: CARD15. RefSeq number(s): NP_071445.1 |
| Spezifität | Human, Mouse, Rat 1040 aa |
Anwendungen
| Applikationshinweise | Peptide ELISA: antibody detection limit dilution 1:64,000. Western Blot: Approx 100kDa band observed in lysates of Human Peripheral Blood Mononucleocytes (calculated MW of 115kDa according to NP_071445.1). Recommended concentration: 0.1-0.3µg/ml. An additional band of unknown identity was also consistently observed at 60kDa. This band was successfully blocked by incubation with the immunising peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported? |
| Reinigung | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Buffer | 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. |
| Lagerung | Aliquot and store at -20°C. Minimize freezing and thawing. |
| Beschränkungen | Nur für Forschungszwecke einsetzbar |
Bilder
| ABIN185703 (0.1µg/ml) staining of Human Peripheral Blood Mononucleocyte lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. |
Publikationen
| Publikationen |
Keshav: "Paneth cells: leukocyte-like mediators of innate immunity in the intestine." in: Journal of leukocyte biology, Vol. 80, Issue 3, pp. 500-8, 2006 (PubMed).
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Alternativen
Alternativen zu Antigen "Nucleotide-Binding Oligomerization Domain Containing 2 (NOD2)", Typ "Antikörper" finden
| Wirte | Kaninchen (42), Maus (9), Ziege (4), Ratte (1) |
| Reaktivitäten | Human (53), Maus (14), Ratte (Rattus) (10), Hund (4), Affe (4), Schwein (3), Rind (Kuh) (2), Kaninchen (2), Huhn (1), Zebrafisch (Brachydanio rerio) (1) |
| Applikationen | Western Blot (WB) (38), Immunfluoreszenz (IF) (23), Durchflusszytometrie (FACS) (17), Immunhistochemie (Paraffinschnitte) (IHC (p)) (16), Immunpräzipitation (IP) (12), Enzyme Immunoassay (EIA) (6), ELISA (4), Immunhistochemie (Gefrierschnitte) (IHC (fro)) (4), Dot Blot (Dot) (1), ELISA (Detektionsantikörper) (1), Immunoelectron Microscopy (IEM) (1), Immunhistochemie (formalinfixierte Schnitte) (IHC (f)) (1) |
| Konjugate | Biotin (3), HRP (3), Alexa Fluor 350 (1), Alexa Fluor 488 (1), Alexa Fluor 555 (1), Alexa Fluor 647 (1), Cy3 (1), Cy5 (1), Cy5.5 (1), Cy7 (1), FITC (1), Gold (1), PE (1), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), RBITC (1) |
| Epitope | C-Term (3), AA 125-140 (1), N-Term (1), internal (1) |




Alternativen