PTPN11 Antikörper (C-Term)

Produktnummer ABIN184604

Der Ziege Polyklonal anti-PTPN11 Antikörper wird verwendet zum Nachweis von PTPN11 in Proben von Human. Er wurde validiert für WB, ELISA, IF und FACS.

Kurzübersicht für PTPN11 Antikörper (C-Term) (ABIN184604)

Target: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Reaktivität: Human

Wirt: Ziege

Klonalität: Polyklonal

Konjugat: Dieser PTPN11 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)

Güteklasse: Verified

Produktdetails anti-PTPN11 Antikörper

Bindungsspezifität: C-Term

Verwendungszweck: SHP2 / PTPN11

Sequenz: YENVGLMQQQ KSFR

Kreuzreaktivität: Rind (Kuh), Human, Maus, Schwein, Ratte

Aufreinigung: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-YENVGLMQQQKSFR, from the C Terminus of the protein sequence according to NP_002825.3.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Western Blot: Approx 70 kDa band observed in Human Muscle lysates (calculated MW of 68.0 kDa according to NP_002825.3). Recommended concentration: 1-3 μg/mL. Primary incubation 1 hour at room temperature.

Peptide ELISA: antibody detection limit dilution 1:128000.

Kommentare:

Immunofluorescence: Strong expression of the protein seen in the nuclei and cytoplasm of HeLa cells. Recommended concentration: 10μg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handhabung: Minimize freezing and thawing.

Lagerung: -20 °C

Informationen zur Lagerung: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Details zu PTPN11

Target: PTPN11 (Protein tyrosine Phosphatase, Non-Receptor Type 11 (PTPN11))

Andere Bezeichnung: PTPN11

Hintergrund: PTPN11, SHP2, protein tyrosine phosphatase, non-receptor type 11 (Noonan syndrome 1), CFC, NS1, BPTP3, PTP2C, SHP-2, PTP-1D, SH-PTP2, SH-PTP3, Noonan syndrome 1, protein-tyrosine phosphatase 2C, protein tyrosine phosphatase, non-receptor type 11, MGC14433

Gen-ID: 5781, 19247, 25622

NCBI Accession: NP_002825, NP_001317366

Pathways: JAK-STAT Signalweg, RTK Signalweg, T-Zell Rezeptor Signalweg, Interferon-gamma Pathway, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Negative Regulation of Hormone Secretion, Carbohydrate Homeostasis, Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, BCR Signaling, Warburg Effekt