D1-Int Antikörper

Produktnummer ABIN165360

Produktdetails anti-D1-Int Antikörper

Target: D1-Int (PsbA)

Reaktivität: Arabidopsis, Cyanobacteria (Nostoc), Nicotiana tabacum

Wirt: Kaninchen

Klonalität: Polyklonal

Applikation: Western Blotting (WB)

Spezifität: Higher plants, cyanobacteria. Antibody reacts with a 32 kDa protein in all tested photosynthetic organisms

Immunogen: synthetic peptide (a. a. 57 – 85) against PsbA2/3 proteins from Synechocystis sp. PCC 6803. The peptide is conservative in D1 protein sequences form all photosynthetic organisms.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: Western blot (1 : 1.000 for ECL)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Lagerung: 4 °C

Referenzen für anti-D1-Int Antikörper (ABIN165360)

Armbruster, Zühlke, Rengstl, Kreller, Makarenko, Rühle, Schünemann, Jahns, Weisshaar, Nickelsen, Leister: "The Arabidopsis thylakoid protein PAM68 is required for efficient D1 biogenesis and photosystem II assembly." in: The Plant cell, Vol. 22, Issue 10, pp. 3439-60, (2010) (PubMed).

del Hoyo, Alvarez, del Campo, Gasulla, Barreno, Casano: "Oxidative stress induces distinct physiological responses in the two Trebouxia phycobionts of the lichen Ramalina farinacea." in: Annals of botany, Vol. 107, Issue 1, pp. 109-18, (2010) (PubMed).

Details zu D1-Int

Target: D1-Int (PsbA)

Hintergrund: D1 is one the major proteins of the reaction centre of photosystem II which carries the Q B -binding site. D1 reduces the reaction centre P680* and provides the ligands to the manganese cluster of the water-oxidizing complex at the lumenal side of thylakoid membrane. D1 protein is synthesized in a precursor form containing a C-terminal extension of 16 amino acid residues in cyanobacteria and 8 – 9 - in eukaryotes. The precursor D1 is co-translationally inserted into the thylakoid membrane and is C-terminally cleaved by CtpA protease (Anbudurai et al., 1994, PNAS 16, 8082 – 8086). D1 protein from higher plants and green algae can be phosphorylated by STN8 kinase (Bonardi et al., 2005, Nature 437, 1179 – 1182, Vaionen et al., 2005, J Biol. Chem. 280, 33679 – 33686).