CD14 Molecule (CD14) Antikörper (Biotin)

Antigen: CD14 Molecule (CD14)

Synonyme: CD14

Klonalität: Monoklonal (61D3)

Wirt: Maus

Reaktivität: Human

Konjugat: Biotin

Produktnummer: ABIN135749

Produktbeschreibung

Weitere Bezeichnung: CD14

Isotyp: IgG1

Klon: 61D3

Beschreibung: Human CD14 CD14 is a 53-55 kDa glycosphosphatidylinositol (GPI)-anchored single chain glycoprotein. It is expressed on monocytes, macrophages, dendritic cells and Langerhans cells. It is also weakly expressed on neutrophils. CD14 appears to be involved in clearance of gram-negative bacteria via its high affinity binding to LPS-LPB complexes.

Anwendungen

Applikationshinweise: Working Dilutions: Flow Cytometry: Purified (1 ug/10^6 cells), Fluorescein (10 L/10^6 cells), R-phycoerythrin (10 L/10^6 cells), Allophycocyanin (10 L/10^6 cells), Spectral Red (10 L/10^6 cells), R-phycoerythrin-Cyanine 5.5 (10 L/10^6 cells), R-phycoerythrin-Cyanine 7 (10 L/10^6 cells), Allophycocyanin-Cyanine 5.5 (10 L/10^6 cells), Allophycocyanin-Cyanine 7 (10 L/10^6 cells). Since applications vary, you should determine the optimum working dilution of the product that is appropriate for your specific need.

Lagerung: The purified (UNLB) antibody is supplied as 0.1 mg of purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.0. No preservatives or amine-containing buffer salts added. Store at 2-8C. The fluorescein (FITC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3. Store at 2-8C. The biotin (BIOT) conjugate is supplied as 100 tests in 1.0 mL PBS/NaN3. Store at 2-8C. The R-PE conjugate and allophycocyanin conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent, store at 2-8C. The low endotoxin/azide-free antibody is supplied as 0.5 mg purified immunoglobulin in 0.2 mL of PBS, aliquot and freeze upon receipt. The Spectral Red (SPRD), R-phycoerythrin-Cyanine 5.5 (R-PE-CY5.5), R-phycoerythrin-Cyanine 7 (R-PE-CY7), Allophycocyanin-Cyanine 5.5 (APC-CY5.5), and Allophycocyanin-Cyanine 7 (APC-CY7) conjugates are supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8C. Do not freeze! Protect conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed. WARNING Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.

Forschungsgebiet: Stammzellen, Angeborene Immunität, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, CD Antigene, Oberflächenrezeptoren der Immunzellen , Apoptose/Nekrose, Immunologie, Inflammation

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Stocks, Albrechtsen, Kerr: "Expression of the CD15 differentiation antigen (3-fucosyl-N-acetyl-lactosamine, LeX) on putative neutrophil adhesion molecules CR3 and NCA-160." in: The Biochemical journal, Vol. 268, Issue 2, pp. 275-80, 1990 (PubMed).