Mdm2 P53 Binding Protein Homolog (Mouse) (MDM2) Antikörper

Antigen: Mdm2 P53 Binding Protein Homolog (Mouse) (MDM2)

Synonyme: HDMX, hdm2, MGC5370, MGC71221, Mdm-2, AA415488, 1700007J15Rik

Klonalität: Polyklonal

Wirt: Kaninchen

Reaktivität: Human, Rind (Kuh), Maus, Ratte (Rattus)

Applikation: ELISA, Western Blot (WB), Immunpräzipitation (IP)

Produktnummer: ABIN129683

Produktbeschreibung

Weitere Bezeichnung: MDM2

Swiss-Prot: P23804

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with asynthetic peptide corresponding to aa 177-195 of mouse MDM2.

Format: Liquid (sterile filtered)

Beschreibung: MDM2 is a nuclear phosphoprotein with an apparentmolecular mass of 90 kD that forms acomplex with the p53 tumor suppressorprotein. Human MDM2 was identified as ahomologous product of the 'murine doubleminute 2' gene (mdm2). The MDM2 geneenhances the tumorigenic potential of cellswhen it is overexpressed and encodes aputative transcription factor. Forming a tightcomplex with the p53 gene, the MDM2oncogene can inhibit p53-mediatedtransactivation. MDM2 binds to p53 andamplification of MDM2 in sarcomas leads toescape from p53-regulated growth control.This mechanism of tumorigenesis parallelsthat for virus-induced tumors in which viraloncogene products bind to and functionallyinactivate p53. Over-expression of the MDM2 oncogene was found in leukemias. Inactivation of tumor suppressor genes leads to deregulated cell proliferation and is a keyfactor in human tumorigenesis. MDM2 interacts physically and functionally with the retinoblastoma (RB) protein and can inhibit itsgrowth regulatory capacity. Both RB and p53 can be subjected to negative regulation by the product of a single cellularprotooncogene. The interference of binding to p53 prevents the interaction of MDM2 and its regulation of the transcriptional activity ofp53 in vivo. Direct association of p53 with the cellular protein MDM2 results in ubiquitination and subsequent degradation of p53.MDM2-p53 complexes were preferentially found in S/G2M phases of the cell cycle. The MDM2 gene is alternatively spliced, producing5 additional splice variant transcripts from the full length MDM2 gene. Four out of five of these alternatively spliced forms (MDM2a-MDMd) are missing substantial portions of the p53 binding domain and retain the acidic domain and the zinc-finger domains. The fifthand smallest transcript (MDM2e) retains the largest spliced region encoding the p53 binding domain, however, it lacks the nuclearlocalization signal, the acidic domain and zinc-finger domains. The alternatively spliced transcripts tend to be expressed intumorigenic tissue, whereas the full-length MDM2 transcript is expressed in normal tissue.

Spezifität: This affinity-purified antibody is directed against mouse MDM2 protein. The product was affinitypurified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against Mouse MDM2 protein. A BLASTanalysis was used to suggest minimal cross reactivity with MDM2 homologues from other sources.

Anwendungen

Applikationshinweise: Recommended Dilutions: Cellular Localization: MDM2 is found in the nucleus and cytoplasm, however, it is expressed predominantly in thenucleoplasm. Interaction with ARF (P14) results in the localization of both proteins to the nucleus. The nucleolar localizationsignals in both ARF and MDM2 may be necessary to allow efficient nucleolar localization of both proteins. ELISA 1:3,000 - 1:12,000 WESTERN BLOT 1:500 - 1:2,000 IMMUNOHISTOCHEMISTRY User Optimized OTHER APPLICATIONS User Optimized. Note: This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditionsfor reactivity should be optimized by the end user. Expect a band approximately 90 kDa in size corresponding to MDM2protein by western blotting in the appropriate cell lysate or extract. See Saucedo, et al (1999) for a discussion on expectedmolecular weights.

Konzentration: 0.51 mg/ml (UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M NaCl, pH 7.2.

Lagerung: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contentsand freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Forschungsgebiet: Krebs, Zellzyklus, Transkriptionsfaktoren

Beschränkungen: Nur für Forschungszwecke einsetzbar