IRS1 Antikörper (pSer307)

Produktnummer ABIN129580

Produktdetails anti-IRS1 Antikörper

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Bindungsspezifität: AA 298-316, pSer307

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser IRS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), ELISA

Produktmerkmale: Concentration Definition: by UV absorbance at 280 nm

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 298-316 of human IRS1 protein.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise: This affinity purified antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to phosphorylated IRS1 protein by western blotting in the appropriate cell lysate or extract.   Minimal reactivity is observed against the non-phosphorylated form of the immunizing peptide.  This antibody is phospho specific for pS307 of IRS1 protein.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Liquid

Konzentration: 0.76 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Referenzen für anti-IRS1 Antikörper (ABIN129580)

Shah, Hunter: "Turnover of the active fraction of IRS1 involves raptor-mTOR- and S6K1-dependent serine phosphorylation in cell culture models of tuberous sclerosis." in: Molecular and cellular biology, Vol. 26, Issue 17, pp. 6425-34, (2006) (PubMed).

Details zu IRS1

Target: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Andere Bezeichnung: IRS1 (IRS1 Produkte)

Synonyme: HIRS-1 antikoerper, G972R antikoerper, IRS-1 antikoerper, IRS1IRM antikoerper, irs1 antikoerper, irsu antikoerper, IRS1 antikoerper, irs-1 antikoerper, irs1-a antikoerper, insulin receptor substrate 1 antikoerper, insulin receptor substrate 2 antikoerper, insulin receptor substrate 1 S homeolog antikoerper, IRS1 antikoerper, Irs1 antikoerper, irs2 antikoerper, irs1.S antikoerper

Hintergrund: Insulin Receptor Substrate 1 (IRS1) acts as a signaling molecule for IL-4, insulin and insulin-like growth factor-I (IGF-I) receptors.  When phosphorylated by the insulin receptor, IRS1 binds specifically to various cellular proteins containing SH2 domains such as phosphatidylinositol 3-kinase p85 subunit or GRB2.  When bound, IRS1 typically activates phos-phatidylinositol 3-kinase p85 subunit.  IRS1 interacts with both the NPXY motif of tyrosine-phos-phorylated IGF1R and the INSR through the PTB domain.  Serine phosphorylation of IRS1 is a mechanism for insulin resistance. Ser-312 phosphorylation inhibits insulin action through disruption of IRS1 interaction with the
Synonyms: HIRS 1 antibody, HIRS1 antibody, Insulin Receptor Substrate 1 antibody, IRS 1 antibody

Gen-ID: 3667

NCBI Accession: NP_005535

UniProt: P35568

Pathways: Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process