Integrin alpha M (ITGAM) Antikörper

Antigen: Integrin alpha M (ITGAM)

Synonyme: CR3A, MO1A, CD11B, MAC-1, MAC1A, SLEB6, MGC117044, CR3, MAC1, Cd11b, Ly-40, Mac-1, Mac-1a, CD11b/CD18, F730045J24Rik, ITGAM, Itgam

Klonalität: Monoklonal (MEM-174)

Wirt: Maus

Reaktivität: Human

Applikation: Durchflusszytometrie (FACS), Immunpräzipitation (IP), Radioimmunoassay (RIA)

Produktnummer: ABIN125683

Produktbeschreibung

Weitere Bezeichnung: CD11b

Immunogen: Human granulocytes

Format: Azide Free

Isotyp: IgG2a  (Passende Sekundärantikörper)

Klon: MEM-174

Beschreibung: CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein thatnon-covalently associates with integrin beta2 subunit (CD18), expression of the CD11bchain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin(Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less onmacrophages. CD11b/CD18 integrin is implicated in various adhesive interactions ofmonocytes, macrophages and granulocytes, facilitating their diapedesis, as well as itmediates the uptake of complement coated particles, serving as a receptor for the iC3bfragment of the third complement component.

Spezifität: The antibody MEM-174 recognizes CD11b antigen (Mac-1 alpha), a 165-170 kDa type Itransmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. TheCD11b mediates neutrophil and monocyte interactions with stimulated endothelium.HLDA VI, WS Code BP 310HLDA VI, WS Code M 18

Anwendungen

Applikationshinweise: Flow Cytometry - Positive control: Peripheral Blood Lymphocytes. Immunoprecipitation - Positive control: GranulocytesCD11b/CD18 transfectants. Application note: Immunoprecipitation with the antibody MEM-174 was successfulyperformed using immuno-affinity sorbents (minicolumns). The final immunosorbent shouldcontain 1-5 mg of antibody/1 ml of sorbent gel (e. g. agarose beads) - It is recommended to stimulate positive control material by LPS 1-2 days before to achievebetter expression of target antigen).

Konzentration: 1 mg/ml

Reinheit: > 95% (by SDS-PAGE)

Reinigung: Purified from hybridoma culture supernatant by protein A-affinity chromatography.

Buffer: Azide free phosphate buffered saline (PBS), approx. pH 7.4, 0.2 µ m filter sterilized.

Lagerung: Store at 2-8 o C. Do not use after expiration date stamped on vial label. For long-term storage aliquot and store at -20 o C. Avoid freeze/thaw cycles.

Forschungsgebiet: Stammzellen, Hämopoetische Vorläufer, Hämatopoetische Stammzellen, Glia Marker, CD Antigene, Oberflächenrezeptoren der Immunzellen

Beschränkungen: Nur für Forschungszwecke einsetzbar

Publikationen

Publikationen: Drbal, Moertelmaier, Holzhauser et al.: "Single-molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement." in: International immunology, Vol. 19, Issue 5, pp. 675-84, 2007 (PubMed).