ELISA: A 1/8,000 dilution of a 1 mg/mL antibody solution will give satisfactory results in a direct ELISA using alkaline phosphatase goat anti-mouse Ig (Jackson Laboratories). Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hRANTES (100 ng/mL), a concentration of 5-7 μg/mL of this antibody is required. Also reported to work in: Immunoprecipitation, Immunohistochemistry on Frozen sections and Western blotting. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Rekonstitution
Restore in sterile water to a concentration of 1.0 mg/mL.
Buffer
PBS without preservatives
Konservierungsmittel
Without preservative
Lagerung
4 °C/-20 °C
Informationen zur Lagerung
The lyophilized antibody is stable at room temperature for one month and for one year from despatch when kept at -20 °C. When reconstituted in sterile water, the antibody is stable for six weeks at 2-8 °C.
RANTES (Regulated-on-activation-normal-T-cell-expressed-and-secreted) is a member of the "intercrine/chemokine" family of cytokines, has been previously shown to be chemotactic for monocytes and T cells of memory/helper phenotype. RANTES has been shown to cause the release of histamine from basophils and activates eosinophils. It also binds to CCR1, CCR3, CCR4 and CCR5. This CC-chemokine enhances antigen-specific T helper (Th) type-1 responses against HIV-1. CC chemokines are small inducible proteins that are related to interleukin 8. In lung adenocarcinoma, RANTES is a predictor of survival while that of interleukin (IL)-8 is associated with a poor prognosis. In several models, tumorigenesis is abolished by RANTES, while it is facilitated by IL-8. Site-directed mutagenesis indicated that regulation of RANTES promoter activity required two nuclear factor (NF)-kappaB response elements but not its activator protein (AP)-1 binding sites.Synonyms: C-C motif chemokine 5, D17S136E, E °CP, Eosinophil-chemotactic cytokine, SCYA5, SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES, cell-specific protein P228