Ziege anti-Rind (Kuh) IgG (Whole Molecule) Antikörper (Biotin)

Details zu Produkt Nr. ABIN118075, Anbieter: Anmelden zum Anzeigen
Antigen
Epitop
Whole Molecule
4681
2536
1879
1066
1006
860
298
294
35
34
31
8
7
7
3
3
2
2
1
1
1
1
1
Reaktivität
Rind (Kuh)
2850
2735
2296
1675
1433
788
613
441
380
379
358
339
291
290
208
138
135
122
41
29
27
25
12
11
9
8
6
5
5
5
4
4
4
4
4
4
4
4
3
3
3
2
2
1
1
1
1
1
Wirt
Ziege
6704
4831
1795
891
609
388
91
55
52
21
8
5
5
5
2
1
1
Klonalität
Polyklonal
Konjugat
Biotin
2236
1872
1737
1356
767
682
519
297
264
223
222
200
156
138
128
110
79
75
69
55
51
48
46
43
40
38
34
34
32
29
27
27
27
27
27
27
27
27
22
22
19
19
19
19
15
15
15
15
15
15
14
12
12
10
10
9
9
9
9
9
9
9
8
8
8
8
8
7
6
6
6
6
5
5
5
4
4
4
4
4
4
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Applikation
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Optionen
Hersteller
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Hersteller Produkt- Nr.
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Immunogen Bovine IgG whole molecule.
Produktmerkmale Molar Ratio: 10-20 BAC molecules per Goat IgG molecule.
Reinigung Immunoaffinity chromatography.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Bovine IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Hintergrund Synonyms: Bovine Immunoglobulin G
Forschungsgebiet Immunology, Secondary Antibodies
Applikationshinweise Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. This product has been assayed against 1.0 g of Bovine IgG in a standardcapture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1:11,000 to 1:45,000 of the reconstitution concentration is suggestedfor this product.

Other applications not tested.
Otimal dilutions are dependent on conditions and should be determined by the user.
Beschränkungen Nur für Forschungszwecke einsetzbar
Rekonstitution Restore with 1.0 mL of deionized water (or equivalent).
Konzentration 2.0 mg/mL
Buffer 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4 contaning 0.01 % (w/v) Thimerisol as preservative and 10 mg/mL Bovine Serum Albumin (BSA, IgG and Protease free) as stabilizer.
Konservierungsmittel Thimerosal (Merthiolate)
Vorsichtsmaßnahmen Biohazard Informations: This product contains thimerosal which is hazardous.
Handhabung Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Lagerung 4 °C/-20 °C
Informationen zur Lagerung Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
Allgemeine Veröffentlichungen Bayer, Wilchek: "Protein biotinylation." in: Methods in enzymology, Vol. 184, pp. 138-60, 1990 (PubMed).